Host cell proteins (HCPs) must be adequately removed from recombinant therapeutics by downstream processing to ensure patient safety, product quality, and regulatory compliance. HCP process clearance is typically monitored by enzyme-linked immunosorbent assay (ELISA) using a polyclonal reagent. Recently, mass spectrometry (MS) has been used to identify specific HCP process impurities and monito...

BACKGROUND Smoking cessation advice from health care providers (HCP) is well-known to be associated with increased quitting. This study sought to understand the extent to which smokers in France who visited a HCP around the time of the implementation of the national ban on smoking received encouragement to quit from a HCP and what kinds of intervention were provided. HCP may have a unique oppor...

Although solid Au is usually most stable as a face-centered cubic (fcc) structure, pure hexagonal close-packed (hcp) Au has been successfully fabricated recently. However, the phase stability and mechanical property of this new material are unclear, which may restrict its further applications. Here we present the evidence that hcp → fcc phase transformation can proceed easily in Au by first-pri...

We present details of the global distribution of high-Ca pyroxene (HCP)-rich sites in the lunar highlands based on the global data set of hyperspectral reflectance obtained by the SELENE Spectral Profiler. Most HCP-rich sites in the lunar highlands are found at fresh impact craters. In each crater, most of the detection points are distributed on the ejecta, rim, and floor of the impact craters ...

By employing first-principles metadynamics simulations, we explore the 300 K structures of solid hydrogen over the pressure range 150-300 GPa. At 200 GPa, we find the ambient-pressure disordered hexagonal close-packed (hcp) phase transited into an insulating partially ordered hcp phase (po-hcp), a mixture of ordered graphene-like H(2) layers and the other layers of weakly coupled, disordered H(...

OBJECTIVE Globally, one third of deaths each year are from cardiovascular diseases, yet no strong evidence supports any specific method of CPR instruction in a resource-limited setting. We hypothesized that both existing and novel CPR training programs significantly impact skills of hospital-based healthcare providers (HCP) in Botswana. METHODS HCP were prospectively randomized to 3 training ...

Administration of hydrocortisone or cortisone to rats has been shown to produce a marked increase in hepatic tryptophan pyrrolase (1, 2) and tyrosine-cY-ketoglutarate transaminase’ (4, 5) activities. Although present evidence suggests that the rise in tryptophan pyrrolase activity involves the synthesis of new protein (6) and the rise in tyrosine transaminase activity may not (7), the mechanism...

OBJECTIVES To investigate the effect of dexamethasone on triggering receptor expressed on myeloid cells-1 (TREM-1). METHODS Wild-type and tumor necrosis factor (TNF (-/-)) mice were pre-treated with saline, dexamethasone, or hydrocortisone and exposed to a lethal infection of Pseudomonas aeruginosa. Mortality and TREM-1 on neutrophil membranes was measured after sacrifice. U937 human monocyti...

Within PrefHer (NCT01401166), patients and healthcare professionals (HCPs) preferred subcutaneous (SC) over intravenous (IV) trastuzumab. We undertook a prospective, observational time and motion study to quantify patients' time in infusion chairs and active HCP time in PrefHer. Patients with HER2-positive early breast cancer received four adjuvant cycles of SC trastuzumab (600 mg fixed dose vi...

conclusions we conclude that intraperitoneal hydrocortisone instillation caused leukocytosis and is thus suggested not to be used in immune-compromised patients. results the study was completed by 120 patients. patients who received intraperitoneal hydrocortisone and hydrocortisone plus bupivacaine had higher white blood cell (wbc) count (p < 0.0001). the patients were similar with respect to d...