Background Cultivation temperature is a key environmental parameter that influences cell growth and recombinant protein production. Recombinant CHO (rCHO) cells are usually cultivated at 37 °C. Although lowering culture temperature below 37 °C decrease specific growth rate, in many cases, the specific production rate, q, of CHO cells was not enhanced by lowering the culture temperature. Unlike ...

This research compared effects of the weed control practice, soil cultivation, and the conventional practice, glyphosate application on weed seedbank, in a vineyard system. The experiment was conducted in a commercial wine-grape vineyard in the Napa Valley of northern California from 2003 to 2005. The annual treatments were ‘‘winter–spring glyphosate,’’ ‘‘spring cultivation,’’ ‘‘fall–spring cul...

OBJECTIVES Recombinant protein production processes in Escherichia coli are usually operated in fed-batch mode; therefore, the elaboration of a fed-batch cultivation protocol in microtiter plates that allows for screening under production like conditions is particularly appealing. RESULTS A highly reproducible fed-batch like microtiter plate cultivation protocol for E. coli in a micro-bioreac...

Ganoderma applanatum belongs to the group of white-rot fungi, due to a well-developed ligninolytic enzyme system. White-rot fungi have attracted great scientific attention in recent years, especially with respect to their enzymatic potential for the bioremediation of persistent pollutants. Contrary to G. lucidum, which medicinal properties, as well as ligninolytic enzyme system have been extens...

This paper describes a microfluidics-based workflow for genetically targeted isolation and cultivation of microorganisms from complex clinical samples. Data sets from high-throughput sequencing suggest the existence of previously unidentified bacterial taxa and functional genes with high biomedical importance. Obtaining isolates of these targets, preferably in pure cultures, is crucial for adva...

The effect of CO2 concentration on the growth and colonial stability of Type 1 Neisseria gonorrhoeae has been investigated. Carbon dioxide at a concentration of 16 per cent in air above flasks incubated in a shaker was effective in supporting growth and 100 per cent colonial stability of Types 1, 1', 2, and 4. Lower CO2 tensions increased the generation time of the strains and were less effecti...

23 An alternative method for cultivation of Lawsonia intracellularis, an obligate intracellular 24 bacterium and causative agent of proliferative enteropathy, was described using Original Space 25 Bag® inflated with a mixture of gas containing 10% hydrogen, 10% carbon dioxide, and 80% 26 nitrogen. The flexibility of this protocol allows for the testing of various environmental 27 conditions for...

One of the major steps toward the industrialization of the microbial product(s) is to optimize the cultivation conditions at the large-scale bioreactor and successfully control the microbial behavior within a large scale production environment. Statistical Design of Experiment was proven to optimize a vast number of microbial processes to achieve robustness and explore possible interactions...

Rapid detection of food-borne pathogens is essential to public health and the food industry. Although the conventional culture method is highly sensitive, it takes at least a few days to detect food-borne pathogens. Even though polymerase chain reaction (PCR) can detect food-borne pathogens in a few hours, it is more expensive and unsatisfactorily sensitive relative to the culture method. We ha...

Nitrogen starvation is an efficient environmental pressure for increasing lipid accumulation in microalgae, but it could also significantly lower the biomass productivity, resulting in lower lipid productivity. In this study, green alga Chlorella sp. A2 was cultivated by using a minimal nitrogen supply strategy under both laboratory and outdoor cultivation conditions to evaluate biomass accumul...