A simple method for isolation of kallikrein from human plasma is described. Before activation of the enzyme with acetone, the plasma was treated with 0.2 M-methylamine at pH 8.2 to inactivate a2-macroglobulin and thus prevent the irreversible binding of the active enzyme to the inhibitor. The enzyme was adsorbed on soya-bean trypsin inhibitor-Sepharose 4B and eluted with 5 mM-NaOH, pH 11.3. It ...

We present an extension of the Hindley-Milner type system that supports a generous class of type constructors called functors, and provide a parametrically polymorphic algorithm for their mapping, i.e. for applying a function to each datum appearing in a value of constructed type. The algorithm comes from shape theory, which provides a uniform method for locating data within a shape. The result...

This paper proposes a novel technique for separating single channel recording of speech mixture using a hybrid of maximum likelihood and maximum a posteriori estimators. In addition, the new algorithm proposes a new approach that accounts for the time structure of the source signals by encoding them into a set of basis filters that are characteristically the most significant. Real time testing ...