نتایج جستجو برای: گاو خالص سیستانیrna seq
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DNA methylation plays key roles in diverse biological processes such as X chromosome inactivation, transposable element repression, genomic imprinting, and tissue-specific gene expression. Sequencing-based DNA methylation profiling provides an unprecedented opportunity to map and compare complete DNA methylomes. This includes one of the most widely applied technologies for measuring DNA methyla...
Roles for transcript leaders in translation and mRNA decay revealed by transcript leader sequencing.
Transcript leaders (TLs) can have profound effects on mRNA translation and stability. To map TL boundaries genome-wide, we developed TL-sequencing (TL-seq), a technique combining enzymatic capture of m(7)G-capped mRNA 5' ends with high-throughput sequencing. TL-seq identified mRNA start sites for the majority of yeast genes and revealed many examples of intragenic TL heterogeneity. Surprisingly...
مقدمه: امروزه شیر گاو یکی از مهم ترین و پرمصرف ترین شیرهای حیوانی مورد تغذیه توسط انسان و یک منبع منحصر به فرد از مواد غذایی برای تمام سنین است. آلودگی شیر به فلزات سنگین مانند سرب و کادمیوم آلوده می شود یک خطر برای انسان به شمار می آید. مسمومیت با سرب و کادمیوم موجب اثرات نامطلوبی بر روی انسان می شود که در دوران کودکی بسیار شایع تر است. این مطالعه به منظور بررسی سطح سرب و کادمیوم در شیر گاو در...
Next-generation RNA sequencing (RNA-seq) technology has been widely used to assess full-length RNA isoform abundance in a highthroughput manner. RNA-seq data offer insight into gene expression levels and transcriptome structure, enabling us to better understand the regulation of gene expression and fundamental biological processes. Accurate quantification of RNA isoforms from RNA-seq data is a ...
The mechanism of toxicity of the leukemogen benzene is not entirely known. This pilot study used RNA-sequencing (RNA-seq) technology to examine the effect of benzene exposure on gene expression in peripheral blood mononuclear cells obtained from 10 workers occupationally exposed to high levels of benzene (≥5 ppm) in air and 10 matched unexposed control workers, from a large study (n = 125) in w...
Recently, investigators have begun evaluating the ability of spiral computed tomography (sequence scan mode-SEQ) to measure coronary calcium. Electron Beam Tomography (EBT) and SEQ studies were performed in 10 women and 23 men, with a mean age of 54+/-9 years. The EBT study was performed within 4 weeks (mean 11+/-4 days) of the SEQ with no clinical interval event (MI, revascularization). The me...
BACKGROUND In previous work, we designed a modified aptamer-free SELEX-seq protocol (afSELEX-seq) for the discovery of transcription factor binding sites. Here, we present original software, TFAST, designed to analyze afSELEX-seq data, validated against our previously generated afSELEX-seq dataset and a model dataset. TFAST is designed with a simple graphical interface (Java) so that it can be ...
Transcriptome analysis is a valuable tool for identification and characterization of genes and pathways underlying plant growth and development. We previously published a microarray-based maize gene atlas from the analysis of 60 unique spatially and temporally separated tissues from 11 maize organs [1]. To enhance the coverage and resolution of the maize gene atlas, we have analyzed 18 selected...
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