For biotransformational studies, Callus of Catharanthus roseus was produced from young shoot tip on Murashige & Skoog medium supplemented with 1.5 mg/litre 2, 4-D and 0.5 mg/litre Kin, solidified by 8 g/litre agar at 25±1°C under complete darkness. The cultures were maintained on the same medium at 25±1 °C in the dark by subculturing after every four weeks. Suspension culture of the plant was p...

The distribution of radiocesium was examined in bamboo shoots, Phyllostachys pubescens, collected from 10 sites located some 41 to 1140 km from the Fukushima Daiichi nuclear power plant, Japan, in the Spring of 2012, 1 year after the Fukushima nuclear accident. Maximum activity concentrations for radiocesium ¹³⁴Cs and ¹³⁷Cs in the edible bamboo shoot parts, 41 km away from the Fukushima Daiichi...

We investigated the regeneration of two Bulgarian sweet pepper cultivars (‘Hebar’ and ‘Stryama’) on solid and liquid culture medium. Liquid cultures were incubated in temporary immersion RITA ® airlift bioreactors. During a period of 60 days, culture in the liquid medium was associated with significantly increased shoot tip growth, number of lateral shoots, percentage of rooted plants and micro...

The vacuolar membrane (tonoplast) contains an abundant intrinsic protein with six membrane-spanning domains that is encoded by a small gene family. Different isoforms of tonoplast intrinsic protein (TIP) are expressed in different tissues or as a result of specific signals. Using promoter-beta-glucuronidase (GUS) fusions and in situ hybridization, we have examined the expression of gamma-TIP in...

Trehalose, an a,a-diglucoside, induced a rapid blackening and death of shoot tips of Cuseuta reflexa (dodder) cultured in vitro. The onset of toxic symptom was delayed if any of the several sugars which support the in vitro growth of Cuscuta was supplied with trehalose. The rate of trehalose uptake or its accumulation in the tissue was not affected by sugar cofeeding. The levels of total and re...

background aloe vera l. is an important pharmaceutical plant from which several medicinal and cosmetic compounds are extracted. aloe is naturally propagated through offset, which is a slow and expensive labor cost method with low economical income. objectives in this study, the effect of different media on shoot proliferation of the shoot tip of aloe vera l. was investigated. materials and meth...