Several studies have shown that nitric oxide (NO) inhibits Na(+) transport in renal and alveolar monolayers. However, the mechanisms by which NO alters epithelial Na(+) channel (ENaC) activity is unclear. Therefore, we examined the effect of applying the NO donor drug l-propanamine 3,2-hydroxy-2-nitroso-1-propylhidrazino (PAPA-NONOate) to cultured renal epithelial cells. A6 and M1 cells were ma...

The structures of alphabeta TCRs bound to complexes of class I MHC molecules and peptide show that the TCRs make multiple contacts with the alpha1 and alpha2 helixes of the MHC. Previously we have shown that the A6 TCR in complex with the HLA-A2/Tax peptide has 15 contact sites on HLA-A2. Single amino acid mutagenesis of these contact sites demonstrated that mutation of only three amino acids c...

Bu deneysel çalışmada, çok ince taneli yüksek fırın cüruflu çimento (Spinor A6) ile stabilize edilmiş düşük plastisiteli killi zeminin bazı mühendislik özelliklerinin belirlenmesi amaçlanmıştır. Öncelikle, %8, %10 ve %12 oranlarında Spinor A6 karıştırılarak elde edilen zemin numunelerinin temel geoteknik özellikleri olarak tespit edilmiştir. Daha sonra, optimum su muhtevası maksimum kuru birim ...

The expression of the Ca2+-dependent epithelial cell adhesion molecule E-cadherin (also known as uvomorulin and L-CAM) in the early stages of embryonic development of Xenopus laevis was examined. E-Cadherin was identified in the Xenopus A6 epithelial cell line by antibody cross-reactivity and several biochemical characteristics. Four independent mAbs were generated against purified Xenopus E-ca...