when a plant is initially transformed, the transgene is present in a heterozygous form. Since white clover is outcrossing and predominantly self incompatible it is necessary to cross primary transgenic plants to produce an BC1 population, then intercross BC1 transgenic plants to produce an F2 population. The F2 population will have a highly heterogeneous genetic background and a variable alleli...

The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% – half strength, and 100% full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A....

Transgenic cell lines of loblolly pine (Pinus taeda L.) were analyzed by a compact laser-tweezers-Raman-spectroscopy (LTRS) system in this investigation. A low power diode laser at 785 nm was used for both laser optical trapping of single transgenic cells and excitation for near-infrared Raman spectroscopy of the nuclei of synchronized cells, which were treated as single organic particles, at t...

Background: Escherichia albertii has been recently isolated from the feces of people with gastroenteritis as a pathogen that causes diarrhea. Due to insufficient information on phenotypic and biochemical characteristics E. albertii, it is difficult distinguish other species Enterobacteriaceae family and, therefore, mistakenly identified coli or even Hafnia alvei. Objective: The present study wh...

Nineveh is one of the main governorates Iraq for sheep ranching. However, in recent years, E.coli has caused many problems and diseases sheep. Therefore, current study was conducted to isolate from their surrounding environment two types breeding systems (open closed) effect system on rates its isolation. During period 1 Feb 30 Apr 2022, 380 samples milk, skin swabs, oral swabs lambs, water, dr...

Esta investigación tuvo por objetivo la caracterización bioquímica y identificación de filogrupos en cepas Escherichia coli, heces terneros con diarrea, mediante el método Clermont. Se recogieron treinta dos muestras ocho rebaños del caserío Tartar Grande, distrito Baños Inca, región Cajamarca, Perú. Mediante crecimiento agar MacConkey-MUG fueron seleccionadas trece caracterizándose bioquímicam...

Abstract Soil microbes perform important functions in nitrogen and carbon cycling the biosphere. Microbial communities rhizosphere enhance plants’ health promote nutrient turnover soil. In this study, we evaluated effects of soil fertilization with organic inorganic fertilizers on abundances distribution genes within maize plants. Our result showed that plants through selected enriched same fun...

Antibiotic-resistance genes carried by coliforms in drinking water is a concerning issue for public health Bangladesh. This research was out to identify and understand the importance of int1 gene spread resistance bacterial antibiotics through consumption contaminated water. A total 31 samples were collected from restaurants ( n = 18), center 9), residences 4) located Chattogram City, The isola...