Leptochloa virgata (L.) P. Beauv. is an annual weed common in citrus groves in the states of Puebla and Veracruz, Mexico limiting their production. Since 2010, several L. virgata populations were identified as being resistant to glyphosate, but studies of their resistance mechanisms developed by this species have been conducted. In this work, three glyphosate-resistant populations (R8, R14, and...

Stretch-receptor (SR) axons form a parallel array of 20 excitatory synapses with target neurons in the crayfish CNS. In each postsynaptic neuron, EPSPs from different SR axons differ significantly in size. These amplitudes are correlated with the segment in which each axon originates and form a segmental gradient of synaptic excitation in individual postsynaptic neurons. These differences might...

We determined the contribution of glutamate receptor subtypes to developing excitatory synaptic transmission in isolated spinal cord of rat embryos. Using electrophysiological and morphological techniques, we studied the pattern of development of synapses between dorsal root afferents and motoneurons in lumbar spinal cords of 15 to Pl-d-old rat embryos. Motoneuron dendritic fields and afferent ...

Many neurons receive excitatory glutamatergic input almost exclusively onto dendritic spines. In the absence of spines, the amplitudes and kinetics of excitatory postsynaptic potentials (EPSPs) at the site of synaptic input are highly variable and depend on dendritic location. We hypothesized that dendritic spines standardize the local geometry at the site of synaptic input, thereby reducing lo...

A key enzyme of the shikimate pathway, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19), is the known target of the widely used herbicide glyphosate. Glyphosate resistance in Amaranthus palmeri, one of the most troublesome weeds in agriculture, has evolved through increased EPSPS gene copy number. The aim of this work was to study the pleiotropic effects of (i) EPSPS increased t...

To study the type of presynaptic calcium channels controlling transmitter release at synaptic connections displaying depression or facilitation, dual whole cell recordings combined with biocytin labelling were performed in acute slices from motor cortex of 17to 22-day-old rats. Layer V postsynaptic interneurons displayed either fast spiking (FS) (n 5 12) or burst firing (BF) (n 5 12) behaviour....

To study the type of presynaptic calcium channels controlling transmitter release at synaptic connections displaying depression or facilitation, dual whole cell recordings combined with biocytin labelling were performed in acute slices from motor cortex of 17- to 22-day-old rats. Layer V postsynaptic interneurons displayed either fast spiking (FS) (n = 12) or burst firing (BF) (n = 12) behaviou...

Golgi cells are poised to integrate multimodal influences by participating in circuits involving granule cells in the cochlear nuclei. To understand their physiological role, intracellular recordings were made from anatomically identified Golgi cells in slices of the cochlear nuclei from mice. Cell bodies, dendrites, and terminals for all seven labeled cells were restricted to the narrow plane ...

Due to ever-increasing global diffusion and related socio-economic implications, the detectionof genetically modified organisms (GMOs) is very important. In this study, we design a plasmidcontaining two genes in mutated form as construct-specific (cp4 epsps) and event-specific(pd35S). It is applied for quantitative-competitor (QC) PCR as a simple and reliable method forthe detection of GM food....

The levels of resistance to glyphosate 13 barnyard grass ( Echinochloa crus-galli ) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined greenhouse and laboratory experiments. Shikimate accumulation fast screening separated regarding glyphosate: susceptible (S) E2, E3, E4, E6 resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, E13. However, fac...