The occurrence of Campylobacter in poultry meat and subsequent antibiotic resistance profiles of the isolates were investigated. The prevalence of Campylobacter in 101 samples was 87.1%. Of these samples, 54.5% contained Campylobacter coli and 45.5% were contaminated with Campylobacter jejuni. Among the strains tested, resistance to ciprofloxacin and nalidixid acid was the most common, followed...

Escherichia albertii is a new enteropathogen of humans and animals. The aim the study was to assess prevalence pathogenicity E. strains isolated in northeastern Poland using epidemiological genomic studies. In 2015–2018, total 1154 fecal samples from children adults, 497 bird droppings, 212 food samples, 92 water 500 lactose-negative coli were tested. A 42 isolated. PCR method suitable for thei...

Seventy cerebrospinal fluid Escherichia coli isolates from infants with neonatal bacterial meningitis (NBM), as submitted to the Netherlands Reference Laboratory for Bacterial Meningitis from 1989 through 1997, were assessed for phylogenetic background and extended virulence genotypes, in comparison with the E. coli reference collection, by using molecular methods. Phylogenetic group B2 signifi...

Cytolethal distending toxins (CDTs) constitute a family of bacterial proteins that enter eukaryotic cells with genotoxic activity leading to cell cycle arrest and apoptosis. CDTs are widespread, having been found in a variety of Gram-negative pathogens with a broad tissue tropism. The recently determined crystal structure of the Haemophilus ducreyi CDT provides a powerful starting point for ana...

Little is known about the molecular pathogenesis of hepatitis and enterocolitis caused by enterohepatic Helicobacter species. Sonicates of the murine pathogen Helicobacter hepaticus were found to cause progressive cell distension, accumulation of filamentous actin, and G(2)/M cell cycle arrest in HeLa cell monolayers. The genes encoding this cytotoxic activity were cloned from H. hepaticus. Thr...

OBJECTIVE To screen a collection of isolates of Haemophilus ducreyi for expression of the cytolethal distending toxin (CDT). METHODS 45 clinical isolates of H ducreyi were screened for cytotoxic activity by examining the effect of culture supernatants on Hela cells. Expression was confirmed using immunoblotting with CDT specific monoclonal antibodies and the presence of the cdt genes determin...

Among 75 urosepsis isolates of Escherichia coli, 29 virulence factor (VF) genes were detected by use of a novel polymerase chain reaction (PCR) assay. Compared with probe hybridization, the PCR assay's specificity was 100% and sensitivity 97.1%. fyuA (yersiniabactin: overall prevalence, 93%), traT (serum resistance, 68%), and a pathogenicity-associated island marker (71%) occurred in most strai...