نتایج جستجو برای: ژن 16s rrna
تعداد نتایج: 50970 فیلتر نتایج به سال:
چکیده- امروزه توجه به تولید محصولات گوشتی بدون تقلب و با هزینه کمتر افزایش یافته است. بنابراین با در نظر گرفتن نگرانی های مربوط به سلامت عمومی اعتقادات مذهبی و مسائل اقتصادی، لازم است اقدامات مناسب جهت ممانعت از این تقلبات به عمل آید. در مطالعه حاضر با استفاده از آزمایش real time pcr به عنوان یک روش سریع، حساس و اختصاصی، به شناسایی و اندازه گیری میزان بافت های مرغی در محصولات گوشتی با برچسب تهی...
background: there is a conserved portion in the 16s rrna gene of bacteria which can be amplified by the universal pcr method. this fragment is 996 bp in length. in this method, only one set of universal primers is used for the amplification of the conserved region of the 16s rrna gene, in common bacterial pathogens. therefore, using the universal pcr method, these bacteria are detectable only b...
The gene encoding the small subunit rRNA serves as a prominent tool for the phylogenetic analysis and classification of Bacteria and Archaea owing to its high degree of conservation and its fundamental function in living organisms. Here we show that the 16S rRNA genes of not-yet-cultivated large sulfur bacteria, among them the largest known bacterium Thiomargarita namibiensis, regularly contain...
PCR-RFLP with nine restriction enzymes was applied to the 16S and 23S rRNA genes of 42 rhizobial and agrobacterial strains to determine the phylogenetic position of Rhizobium galegae and increase the understanding of the evolution of ribosomal operons. The strains were selected based on previous phylogenetic studies. PCR primers were designed so that they amplified a 2.3 kb fragment of the 23S ...
Identification and quantification of phylogenetically defined bacterial populations in the environment are often performed using molecular tools targeting 16S rRNA. Fluorescence in situ hybridization has been used to monitor the expression and processing of rRNA by targeting the 3' tail of precursor 16S rRNA. To expand this approach, we employed reverse transcription of total RNA using primer S...
Denaturing gradient gel electrophoresis (DGGE) of DNA fragments obtained by PCR amplification of the V2-V3 region of the 16S rRNA gene was used to detect the presence of Lactobacillus species in the stomach contents of mice. Lactobacillus isolates cultured from human and porcine gastrointestinal samples were identified to the species level by using a combination of DGGE and species-specific PCR...
مقدمه: جنس نوکاردیا جزو اکتینومیستهای هوازی هستند که یک گروه بزرگ از باکتریهای ساکن خاک را شامل میشوند، که در جهان پخش شدهاند. در این مطالعه، با استفاده از آزمونهای تشخیصی رایج، مرسوم و مولکولی گوناگون، چندین ایزولههای نوکاردیا جداسازی و تشخیص داده شد. مواد و روش ها: در این پژوهش، برای جداسازی از خاک، روش Slip-buried و برای استخراج DNA، روش Microwave oven استفاده شد و در این روشها، سو...
INTRODUCTION Buruli ulcer (BU) caused by Mycobacterium ulcerans is effectively treated with rifampicin and streptomycin for 8 weeks but some lesions take several months to heal. We have shown previously that some slowly healing lesions contain mycolactone suggesting continuing infection after antibiotic therapy. Now we have determined how rapidly combined M. ulcerans 16S rRNA reverse transcript...
Pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. A polymerase chain reaction (PCR) assay was developed using primers derived from conserved part of 16S-23S rRNA gene. The PCR amplified a fragment size of 0.7 kb using DNA from nine avian P. multocida isolates...
Introduction: Mycoplasmas are one of the most serious contaminants of cellular cultures and their biological productions. Mycoplasma diagnosis is conducted on the basis of culture and molecular methods. These methods are different from each other in terms of accuracy, reliability, and sensitivity. This study aimed to trace the mycoplasma contaminations in culture samples using 16S rRNA specific...
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