The scientific community is still looking for a bright, stable red fluorescent protein (FP) as functional as the current best derivatives of green fluorescent protein (GFP). The red FPs exploit the reduced background of cells imaged in the red region of the visible spectrum, but photophysical short comings have limited their use for some spectroscopic approaches. Introduced nearly a decade ago,...

BACKGROUND Family physicians (FPs) have an opportunity to diagnose pigmented lesions early with a timely biopsy. OBJECTIVE To assess current biopsy practices used by FPs in diagnosing melanoma. METHODS A computer-generated random sample of 200 practicing FPs from large and small communities in Southwestern Ontario was identified from the College of Physicians and Surgeons of Ontario physici...

BACKGROUND Misuse of alcohol is second only to tobacco as a leading cause of preventable death in Australia. There is an opportunity in family practice to detect problems and intervene with people at risk of alcohol-related harm before complications occur. However, family practitioners (FPs) report low levels of confidence in managing patients with drinking problems. The aim of this study was t...

In order to track the assembly of murine leukemia virus (MLV), we used fluorescence microscopy to visualize particles containing Gag molecules fused to fluorescent proteins (FPs). Gag-FP chimeras budded from cells to produce fluorescent spots, which passed through the same pore-size filters and sedimented at the same velocity as authentic MLV. N-terminal myristylation of Gag-FPs was necessary f...

OBJECTIVE To evaluate a curriculum for teaching family physicians (FPs) in rural and underserviced areas about children's mental health, and to evaluate a collaborative model of teaching using child psychiatrists and FPs. METHODS A child psychiatrist and a rural FP provided training to rural FPs in attention-deficit/hyperactivity disorder (ADHD) and disruptive behaviour disorders (DBDs). Trai...

A large number of false positives (FPs) generated by computer-aided detection schemes is likely to distract radiologists’ attention and decreases their interpretation efficiency. Therefore, it is desirable to reduce FPs as many as possible to increase the detection specificity while maintaining the high detection sensitivity. In this paper, several features are extracted from the projected imag...

PURPOSE Continuous and rapid eye movement causes significant intraframe distortion in adaptive optics high resolution retinal imaging. To minimize this artifact, we developed a high speed adaptive optics line scan confocal retinal imaging system. METHODS A high speed line camera was employed to acquire retinal image and custom adaptive optics was developed to compensate the wave aberration of...

INTRODUCTION One of the key strategies for marketing new drugs to physicians is personal selling by pharmaceutical sales representatives (PSRs). GOAL The aim of this study was to determine which features of PSR's are most valued by Slovenian family physicians (FPs). METHODS We performed a cross-sectional observational postal survey in FPs. We sent the invitation for cooperation in the study...

BACKGROUND Emotion dysregulation has been implicated in the negative outcomes following trauma exposure. A proposed biomarker of emotion dysregulation, respiratory sinus arrhythmia (RSA), has demonstrated associations with trauma-related phenomena, such as the fear-potentiated startle (FPS) response. The current study aimed to examine the prospective association between emotion dysregulation an...