The PCR-based DNA fingerprinting method called the methylation-sensitive amplified fragment length polymorphism (MS-AFLP) analysis is used for genome-wide scanning of methylation status. In this study, we developed a method of fluorescence-labeled MS-AFLP (FL-MS-AFLP) analysis by applying a fluorescence-labeled primer and fluorescence-detecting electrophoresis apparatus to the existing method o...

In this paper, utilization of clustering algorithms for data fusion in decision level is proposed. The results of automatic isolated word recognition, which are derived from speech spectrograph and Linear Predictive Coding (LPC) analysis, are combined with each other by using fuzzy clustering algorithms, especially fuzzy k-means and fuzzy vector quantization. Experimental results show that the ...

Using in silico amplified fragment length polymorphism (AFLP) fingerprints, we explore the relationship between sequence similarity and phylogeny accuracy to test when, in terms of genetic divergence, the quality of AFLP data becomes too low to be informative for a reliable phylogenetic reconstruction. We generated DNA sequences with known phylogenies using balanced and unbalanced trees with re...

Three strains of facultatively anaerobic thermotolerant and moderately halophilic bacteria were isolated from two Jordanian hot spring locations. The novel Bacillus strains were short rods, Gram-positive, motile and spore formers. The strains were capable of anaerobic (but not aerobic) growth at 63C, moderately halophilic, able to grow at NaCl concentration range of 12 15 % and at temperatures ...

Abstract In recent years, tan spot of wheat caused by the fungus Pyrenophora tritici-repentis has become more prevalent in Oklahoma. Experiments were conducted to investigate race structure, disease symptoms and genetic variability P. isolates collected from winter over three decades. Race determination was for 16 based on expression necrosis and/or chlorosis produced differentials. Variability...