BACKGROUND Although high-performance liquid chromatography (HPLC) is the commonly used method for the analysis of retinol in biological samples, simple and rapid test kits are available. OBJECTIVES This study compared a rapid test kit (ICHECK Fluoro®) to HPLC for the assessment of serum retinol concentrations. METHODS For the analysis by HPLC, sample preparation included standard deproteini...

The HPLC/MS system, in which a monolithic silica capillary column is directly connected to an electronspray-ionization mass spectrometer, showed superior performance at high mobile phase linear velocity. A two-dimensional (2D) HPLC/MS system was established, using an ion-exchange particle-packed capillary column at the first dimension and a monolithic silica capillary column at the second dimen...

As one of the widely used veterinary macrolide antibiotics, the environmental risks of tylosin (TYL) received more and more attention. The purpose of the present study was to develop a modified, rapid and precise RP-HPLC method with PDA detection for the determination of the macrolide antibiotic tylosin in liquid samples. The proposed two HPLC methods offered short retention time (< 6.8 min), h...

Previously, we developed a reversed-phase HPLC method compatible to high performance liquid chromatography diffuse reflectance Fourier-transform infrared (HPLC-FTIR) thermospray interface for the analysis of dyes. Dye separation achieved with a mixed-mode (SCX-ODS) column using a small gradient (90 to 80% water with acetic acid) and pH 3.25; 10 to 20% acetonitrile was considered to be suitable ...

A multicomponent pharmaceutical that contains loratadine, paracetamol, and pseudoephedrine was quantified using HPLC-PDA. The three analytes were well-separated in the dosage form on a C-18 column gradient mobile phase. quality by design strategy followed to achieve challenging separation. Screening optimization steps carried out investigate effect of many factors studied responses with minimum...

Rape pollen has always been considered as a research hotspot in health foods and pharmaceuticals due to its abundance of natural active ingredients. In this work, compound with antioxidant activity was directly isolated from the methanol extract rape using two-step procedure, under supervision online HPLC−1,1-diphenyl-2-picrylhydrazyl (HPLC-DPPH) detection. Firstly, HPLC−DPPH detection used ide...

Abstract A homochiral metal–organic framework (MOF) comprising [Co(L)(bpe)2(H2O)2]·H2O was prepared using (1R,2R)-(−)-1,2-cyclohexanedicarboxylic acid (H2L) and 1,2-bis(4-pyridyl)-ethylene as organic ligands. The MOF explored chiral stationary phase (CSP) for high-performance liquid chromatography (HPLC) separation of racemates. Nine racemates including naphthol, alcohol, diol, amine, ketone, e...

zataria multiflora boiss is used in traditional folk remedies as antiseptic, analgesic, carminative, anthelmintic medication. the main components of zataria multiflora are phenolic compounds such as thymol and carvacrol. the aim of this study was developed a simple and rapid method for determination of the thymol in different extracts of zataria multiflora by hplc method. the dried aerial parts...