Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) expressed on a variety of DCs, is a C-type lectin receptor that recognizes glycans on a diverse range of pathogens, including parasites. The interaction of DC-SIGN with pathogens triggers specific signaling events that modulate DC-maturation and activity and regulate T-cell activation by DCs. In this work we evaluate whether F. hepa...

A cDNA encoding a 16.5-kDa protein termed FhTP16.5 was identified by immunoscreening of a cDNA library from Fasciola hepatica adult flukes using pooled sera from rabbits infected with F. hepatica for 4 weeks. Quantitative reverse transcriptase PCR (qPCR) analysis revealed that FhTP16.5 is not expressed in unembryonated eggs. It is poorly expressed in miracidia and highly expressed at the juveni...

Indonesian thin-tail (ITT) sheep resist infection by Fasciola gigantica by an immunological mechanism within 2 to 4 weeks of infection yet are susceptible to F. hepatica infection. Studies of ITT sheep show that little liver damage occurs following F. gigantica infection, suggesting that the invading parasites are killed within the peritoneum or shortly after reaching the liver. We investigated...

Understanding genetic structure and status of genetic variation of the Fasciola gigantica and F. hepatica isolates from different hosts, has important implications for epidemiology and effective control of fasciolosis. The aim of the present work was to study the molecular characterization of Fasciola gigantica and F. hepatica isolates collected from cows and sheep, using the random amplified p...

Digestive parasites are a constraint on buffalo productivity. Data regarding the prevalence of parasitic diseases in buffaloes reported developed countries, but Romania, they missing. The aim this study was to evaluate internal raised households and farms from Romania. Faeces samples were collected 180 different ages (calves: 0-6 months; juveniles: 6-30 adults:> 30 months). faecal processed ...

Livestock infection by the parasitic fluke Fasciola hepatica causes major economic losses worldwide. The excretory-secretory (ES) products produced by F. hepatica are key players in understanding the host-parasite interaction and offer targets for chemoand immunotherapy. For the first time, subproteomics has been used to compare ES products produced by adult F. hepatica in vivo, within ovine ho...

AIM The aim of this study was to investigate the prevalence of human and animal fascioliasis in Ilam Province, Iran. BACKGROUND Fascioliasis, caused by Fasciola hepatica, is one of the most important zoonotic diseases. Snails are an intermediate host. Human infection with the parasite can led to hypertrophy and hyperplasia in bile duct. It also economic importance and further information is e...

Recently, the prevalence of Fasciola hepatica in some areas has increased considerably and the availability of a vaccine to protect livestock from infection would represent a major advance in tools available for controlling this disease. To date, most vaccine-target discovery research on this parasite has concentrated on proteomic and transcriptomic approaches whereas little work has been carri...

INTRODUCTION We recently reported the biochemical characterization of a novel Fasciola hepatica recombinant antigen termed FhSAP2, which has previously shown to elicit protection to F. hepatica infection in rabbits. Further we reported that intramuscular (IM) injections of BALB/c mice with a pFLAG-CMV(-2) vector carrying cDNA encoding for FhSAP2 (cDNA-FhSAP2) induce high levels of immune respon...