the aim of this study was to determine the quality of cumulus-oocyte-complexes (cocs) and the effects of bovine serum albumin (bsa) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. cocs were collected from slaughterhouse buffalo ovaries by aspiration method. only normal grades cocs were matured for 48 hours in tcm-199 media. two groups were created: one for the ...

The relationship between the molecular structure of dietary flavonoids and their affinities for bovine serum albumin (BSA) were fully investigated by fluorescence titration analysis. The binding process with BSA was significantly affected by the molecular structure of flavonoids under study. The methylation of hydroxyl group in flavonoids enhanced their binding affinities for BSA by 1 to 794 ti...

dietary flavonoids show beneficial effects in the prevention of chronic diseases. however, flavonoid bioavailability is poor, probably due to their interaction with serum albumins. in the current work, the binding interactions of eight related flavonoids, sharing a similar core structure, with bovine serum albumin (bsa) were investigated by fluorescence spectroscopy. the binding affinities of t...

1-Methyl-3-(2-oxyethyl)-1H-Imidazol-3-ium-Borate Sulfonic Acid ([MOEI]-BSA) was easily prepared and used as a new and highly efficient solid acid catalyst for the synthesis of benzimidazole derivatives with high isolated yields. Various substituted benzimidazoles were synthesized by a combination of o-phenylenediamines and aldehydes in the presence of [MOEI]-BSA with excellent yields in water a...

The effects of lipid-rich bovine serum albumin (LR-BSA) on the development of porcine blastocysts produced in vitro were examined. Addition of 0.5 to 5 mg/ml LR-BSA to porcine blastocyst medium (PBM) from Day 5 (Day 0 = in vitro fertilization) significantly increased the hatching rates of blastocysts on Day 7 and the total cell numbers in Day-7 blastocysts. When Day-5 blastocysts were cultured ...

Effect of bovine serum albumin (BSA} on the absorption of sulfonamides was studied employing in situ recirculating perfusion method in the rat. The absorption rate constant of sulfamethoxazole was greatly decreased by addition of BSA to the perfusion solution. But, the decreasing effect of BSA on the absorption rate was diminished after removing BSA from perfusion solution. The decreasing effec...

Interaction between bovine serum albumin (BSA) and phosphorus heterocycles (PHs) was studied using multi-spectroscopic techniques. The results indicated the high binding affinity of PHs to BSA as it quenches the intrinsic fluorescence of BSA. The experimental data suggested the fluorescence quenching mechanism between PHs and BSA as a dynamic quenching. From the UV-vis studies, the apparent ass...

Three dietary monosaccharides, (glucose, fructose, and ribose), have different rates of protein glycation that accelerates the production of advanced glycation end-products (AGEs). The present work was conducted to investigate the effect of ferulic acid (FA) on the three monosaccharide-mediated protein glycations and oxidation of BSA. Comparing the percentage reduction, FA (1-5 mM) reduced the ...