The grcat potential value of electron microscopy for biological research was often strcsscd and quickly understood in the middle 1930s, at the time when thc first laboratory models of transmission electron microscopes were being built by Ruska, yon Borrics (1, 2), and Marton (3, 4). The reasons were clear: the new instruments were expected to have a resolving power 40-50 times higher than the b...

OBJECTIVES The aim of this study was to develop a novel method for automated quantification of cell-free hemoglobin (fHb) based on the HI (Roche Diagnostics). METHODS The novel fHb method based on the HI was correlated with fHb measured using the triple wavelength methods of both Harboe [fHb, g/L = (0.915 * HI + 2.634)/100] and Fairbanks et al. [fHb, g/L = (0.917 * HI + 2.131)/100]. fHb conce...

Two models of capillary blood prothrombin time (PT) monitoring systems were evaluated for analytical performance and then compared with two routine PT systems using the reference manual technique and a high-sensitivity thromboplastin. Two sets of 60 and 80 plasmas were analyzed from anticoagulated patients stabilized over 3 months in an INR range 2-3.5 for therapy. Capillary PT determination wa...

We performed a comparative analysis between Roche Amplicor HIV-1 DNA test and CAPTAQ assay for the detection of HIV in 830 dried blood spot (DBS) pediatric samples collected in Mozambique. Our results demonstrated no statistical difference between these assays. The CAPTAQ assay approached nearly 100% repeatability/accuracy. The increased throughput of testing with minimal operator interference ...

Analysis of minimal residual disease (MRD) can predict outcome in childhood acute lymphoblastic leukemia (ALL). We applied a chemiluminescent methodology in 20 children with ALL. We detected MRD at different time-points throughout the follow-up of our patients, concluding that chemilumi-nescent detection of MRD is a reliable, safe and sensitive method. Recent prospective studies clearly demonst...

ASCO’s conflict of interest policy, please refer to the Author Disclosure Declaration and the Disclosures of Potential Conflicts of Interest section in Information for Contributors. Employment or Leadership Position: None Consultant or Advisory Role: Alberto F. Sobrero, Bayer (C), Amgen (C), Roche (C), Merck Serono (C), Astra Zeneca (C), sanofi-aventis (C) Stock Ownership: None Honoraria: Alber...

Disclosures: Kathleen I. Pritchard: Consultant/advisory role: Novartis, Roche, AstraZeneca, GlaxoSmithKline, Amgen, Pfizer; Honoraria: GlaxoSmithKline, Amgen, Novartis, Roche, AstraZeneca, Pfizer; Expert testimony: AstraZeneca, Novartis, GlaxoSmithKline. The content of this article has been reviewed by independent peer reviewers to ensure that it is balanced, objective, and free from commercial...