Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IRI measurement in rats can be made more sensitive, accurate, and practical, as demonstrated by a new modified enzyme-linked immunosorbent assay (ELISA). It is characterized by indirect binding of an anti-insulin antibody by an antiglobulin antibody and uses the principle of competitive saturation. In this ELI...

BACKGROUND Acute ischemia reperfusion injury (IRI) results in muscle atrophy and functional loss. Although studies have shown that stem cells can improve muscle function in chronic ischemia caused by vascular diseases, none investigated whether stem cells can improve muscle function following acute IRI. The primary purpose of this study was to determine whether transplantation of muscle progeni...

Transplantation of mesenchymal stem cells (MSCs) has been proven to be effective in attenuation of ischemia-reperfusion injury (IRI), which was related to the paracrine effects of MSCs. In this study, we used hepatocyte growth factor (HGF) siRNA to knock down the HGF expression of bone marrow-derived mesenchymal stem cells (BM-MSCs); Transwell chambers and 6-well plates were then used to build ...

Renal ischemia-reperfusion injury (IRI) is a common cause of acute kidney injury. Toll-like receptor 4 (TLR4) mediates sterile inflammation following renal IRI. Heat shock protein 90 (Hsp90) inhibition is a potential strategy to reduce IRI, and AT13387 is a novel Hsp90 inhibitor with low toxicity. This study assessed if pre-treatment with AT13387 could reduce renal IRI and established if the me...

Recent studies have shown the remarkable gender differences in the susceptibility or expression of many diseases. The mechanism underlying the gender differences is unclear. In the present study, we evaluated the effects of gender differences and different ischemia time on the renal ischemia-reperfusion injury (IRI). The IRI was induced in the bilateral kidneys of 156 male and 30 female BALB/c ...

Repressor and activator protein (Rap1) directly regulates nuclear factor-κB (NF-κB) dependent signaling, which contributes to hepatic IRI. We here intended to investigate the effect of Rap1 in hepatic ischemia reperfusion injury (IRI) and to explore the underlying mechanisms. The association of Rap1 expression with hepatic inflammatory response were investigated in both human and rat liver tran...