OBJECTIVES The aim of the study was to develop a simple assay for rapid detection of the mcr-1 gene, recently identified as a source of plasmid-mediated acquired resistance to polymyxins in Enterobacteriaceae. METHODS A SYBR Green-based real-time PCR assay was designed for detection of the mcr-1 gene. This assay was applied to cultured bacteria and to spiked human and cattle stools. RESULTS...

Colistin resistance was investigated in 1,696 isolates collected from 2007 to 2014 within the frame of the French livestock antimicrobial resistance surveillance programme. The mcr-1 gene was detected in all commensal Escherichia coli isolates with a minimum inhibitory concentration to colistin above the 2 mg/L cut-off value (n=23). In poultry, mcr-1 prevalence was 5.9% in turkeys and 1.8% in b...

The emergence of Enterobacteriaceae strains resistant to both colistin and carbapenem, which harbor the mobile resistance (mcr) gene, has become a significant clinical public health concern. aim this study was identify with co-resistance carbapenem gain further understanding antimicrobial characteristics these in South Korea. Bacterial identification performed using MALDI Biotyper Vitek2 compac...

Cognitive disorders represent a leading cause of disability in the aging population, of which dementia has the highest global burden. Early signs of dementia such as slow gait and memory complaints are known to present well before the overt manifestation of the disease. Motoric cognitive risk (MCR) syndrome characterized by the simultaneous presence of gait disturbances and memory complaints in...

In document processing, segmentation is done to uniquely identify each foreground connected region of an image by specifying its minimum containing rectangle (MCR). MCR is the rectangle with minimum dimensions that completely encloses a geometric pattern. In this paper, we present a two-step MCR detection algorithm and its parallelization method. The first step determines the boundary of each c...

Background Due to increasing colistin usage, the dissemination of the colistin-resistant gene mcr-1 has been increasingly investigated. The aim of this study was to determine whether a traveler on a short-term international trip to a developing country could bring mcr-1 back to their home country. Materials and methods Thirty-four travel events from Japan to Vietnam encompassing 19 travelers ...

The mcr-1 gene was found in 11 isolates from a Portuguese Salmonella collection (n = 1,010; 58 serotypes; 2002-15) of clinical samples, foodstuff, food-animals and water. Mcr-1 has been located on different plasmids (IncX4/IncHI2) in pig-associated multidrug-resistant, copper-tolerant S.1,4,[5],12:i:-/ST34 and S. Rissen/ST469 clones from human and pork products since at least 2011. Our data hig...

Medical case retrieval (MCR) is information retrieval in a collection of medical case descriptions, where descriptions of patients’ symptoms are used as queries. We apply known text retrieval techniques based on query and document expansion to this problem, and combine them with new algorithms to match queries and documents with Medical Subject Headings (MeSH). We ran comprehensive experiments ...

Dear Editor, Following the recent description of transmissible plasmid-mediated colistin resistance encoded by mcr-1 in clinical and veterinary Escherichia coli and Klebsiella pneumoniae isolates in China,1 several groups have reported mcr-1 in colistin-resistant isolates from humans and food animals across Asia2–4 to Europe.5–8 It is evident that mcr-1 has disseminated globally. We performed a...