BACKGROUND Currently, no published data exist about the gonadal function of children born after ICSI. To evaluate potential risk of testicular seminal dysfunction in boys born to fathers with compromised spermatogenesis, serum inhibin B (as a marker for spermatogenesis) was assessed. METHODS We recruited 50 pubertal adolescents from the oldest cohort of infants born following ICSI. Cross-sect...

Background: This study was designed to respond to the controversy regarding the necessity of oocyte activation after the ICSI procedure for normal embryo development in sheep. Materials and Methods: The effect of chemical stimulation with either 5mM ionomycin (I) for 5 minutes or ionomycin + 2 mM 6-dimethylaminopurine (6-DMAP) for 3 hours on the efficiency of ICSI, was compared in six experimen...

Intracytoplasmic sperm injection (ICSI) is a component of infertility treatment often employed when conventional in vitro fertilization is unlikely to be successful. Despite good clinical results with ICSI, the procedure is typically associated with degeneration of a significant percentage (approximately 10%) of the treated oocytes. The cause of this degeneration remains unclear. Speculation th...

Assisted reproductive technology with intracytoplasmic sperm injection (ICSI) is becoming an international panacea for couples struggling with infertility. The increasing popularity of these techniques and the data generated has given us a better understanding of the efficacy, consequences and costs of these procedures. There still remain many unanswered questions and controversies surrounding ...

In pigs, intracytoplasmic sperm injection (ICSI) efficiency is still poor. The inadequate decondensation of the sperm chromatin, its transformation into the male pronucleus (MPN) together with the subsequent inability to activate the oocyte, seem to be the main causes of the low ICSI efficiency. In order to improve the MPN formation we took two different approaches. On the one hand, the in vitr...

An ultrasonographic evaluation of the endometrium was performed in 158 patients undergoing ovarian stimulation for an in-vitro assisted reproduction programme. Endometrial thickness was evaluated in 109 patients undergoing in-vitro fertilization (IVF) for female indications and in 49 patients undergoing intracytoplasmic sperm injection (ICSI) for male indications. The maximal endometrial thickn...

Over the years, IVF/ICSI protocols have continued to evolve with efforts to improve outcomes. As a result, treatment success may be related to certain procedural factors, including number of embryos transferred and stage at which they are transferred. This review aims to assess the safety and effectiveness of IVF/ICSI in comparison to spontaneous conception and less invasive ARTs and the impact...

Background: In the past 22 years, intracytoplasmic sperm injection (ICSI) on human oocytes become a successful method of treatment for most categories of infertility. But there are real concerns that possible malformations among ICSI infants are still not fully recognized. Despite the success of ICSI, the risk of major birth defects due to various parental factors or treatment may increase. We ...

ICSI was ®rst applied in cases of severe male factor infertility in which conventional IVF would be expected to fail or to give poor outcomes (Van Steirteghem et al., 1993). More recently, however, ICSI has been increasingly used in cases in which conventional IVF would not necessarily be inef®cient. In such cases, ICSI is often preferred because of its reliability in terms of achieving high fe...