A perforated-patch whole-cell recording method was used to determine whether nitric oxide signaling participates in acetylcholine (ACh)-induced regulation of basal L-type Ca2+ current (ICa,L) in cat atrial myocytes. Exposure to 1 microM ACh for 2 min inhibited basal ICa,L (-21 +/- 3%), and withdrawal of ACh elicited rebound stimulation of ICa,L above control (80 +/- 13%) (n = 23). Stimulation o...

BACKGROUND We have previously shown major differences in beta-adrenergic and muscarinic modulation of L-type calcium currents (ICa) in newborn and adult rabbit heart. However, little is known about developmental changes in modulation of ICa by phosphodiesterases (PDEs), which also regulate intracellular cAMP concentration by its hydrolysis. METHODS AND RESULTS Enzymatically isolated adult and...

The effects of changing the intracellular Ca concentration ([Ca]i) on the calcium current (iCa) were studied in isolated single ventricular cells of the guinea-pig. [Ca]i was varied by an intracellular perfusion technique using a suction pipette. iCa measured from internally perfused cells at a pCa lower than 9.0 was dependent on the extracellular Ca concentration ([Ca]o). Increasing [Ca]o from...

BACKGROUND AND OBJECTIVE The relationship between EEG source signals and action-related visual and auditory stimulation is still not well-understood. The objective of this study was to identify EEG source signals and their associated action-related visual and auditory responses, especially independent components of EEG. METHODS A hand-moving-Hanoi video paradigm was used to study neural corre...

Severe internal carotid artery (ICA) stenosis is a common cause of cerebrovascular accident (CVA) in middle-aged patients. Contralateral carotid occlusion (CCO) in patients with severe ICA stenosis is associated with high risk of CVA. Carotid endarterectomy (CEA) is associated with more complications in patients with CCO than those without CCO. In this study, we present the case of a 61-year-ol...

Geometric algorithms for linear independent component analysis (ICA) have recently received some attention due to their pictorial description and their relative ease of implementation. The geometric approach to ICA has been proposed first by Puntonet and Prieto [6] in order to separate linear mixtures. One major drawback of geometric algorithms is, however, an exponentially rising number of sam...

Most ICA algorithms are sensitive to outliers. Instead of robustifying existing algorithms by outlier rejection techniques, we show how a simple outlier index can be used directly to solve the ICA problem for super-Gaussian source signals. This ICA method is outlier-robust by construction and can be used for standard ICA as well as for overcomplete ICA (i.e. more source signals than observed si...

The kinetics of activation and inactivation of the inward calcium current (ICa) in morphologically undifferentiated and differentiated neuroblastoma X glioma hybrid cells of the clone 108CC15 were studied by the suction pipette technique for internal perfusion and voltage clamping. Potassium currents were eliminated by internal perfusion of the cells with a K+-free solution. Activation of ICa f...

This paper evaluates the performance of some major Independent Component Analysis (ICA) algorithms like Hyv ̈arinen’s fixed point algorithm, Pearson based ICA algorithm and OGWE (Optimized Generalized Weighted Estimator) ICA algorithm in a biomedical blind source separation problem. Independent signals representing Fetal ECG (FECG) and Maternal ECG (MECG) generated and then mixed linearly to sim...

The regulation of calcium channel currents (ICa) induced by daily stimulation (1 hr) with 10 microM glutamate was studied in full differentiated hippocampal cells in culture. We report a specific enhancement of the high-voltage-activated current type (HVA ICa) ongoing over days. The density of HVA ICa increased about twofold after the second glutamate session, and this enhancement was still obs...