A forward mutagenesis system based on the acquisition of mutations that inactivate the thymidylate synthase gene (TMS) and confer a trimethoprim resistant (Tmpr) phenotype was developed and utilized to study transcription-mediated mutagenesis (TMM). In addition to thyA, Bacillus subtilis possesses thyB, whose expression occurs under conditions of cell stress; therefore, we generated a thyB- thy...

To estimate how many genes produce multiple protein isoforms, we electrophoresed proteins from MCF7 and MDA-MB231 (MB231) human breast cancer cells in SDS-PAGE and excised narrow stripes of the gel at the 48kD, 55kD and 72kD. Proteins in these stripes were identified using liquid chromatography and tandem mass spectrometry. A total of 765, 750 and 679 proteins from MB231 cells, as well as 470, ...

[Purpose] This study was conducted to examine the effect of pelvic stabilization exercise and 3D equipment exercise on adult women with Functional Leg-Length Discrepancy (FLLD). [Subjects and Methods] Twenty female students in their 20's having FLLD without Structural Leg Length Discrepancy were selected. Exercise was performed for 50 min per session, three times a week, for six weeks. The Pelv...

Polarization is an important characteristic of electromagnetic (EM) waves, and efficient manipulations over EM wave polarizations are always desirable in practical applications. Here, we review the recent efforts in controlling light polarizations with metamaterials, at frequencies ranged from microwave to visible. We first presented a 4 × 4 version transfer matrix method (TMM) to study the sca...

Since the discovery of the mirror neuron system in the 1980s, little, if any, research has been devoted to the study of interactive motor tasks (Goldman, 2012). Scientists interested in the neuropsychophysiological markers of joint motor action have relied on observation paradigms and passive tasks rather than dynamic paradigms and interactive tasks (Konvalinka and Roepstorff, 2012). Within thi...

Robust stochastic modeling of speech is an important issue for the performance of practical applications. The Gaussian mixture model, GMM, is widely used in speaker ID, but its performance would get limited in the presence of unseen noise and distortions. Such noisy data, referred to as ”outliers” for the original distribution, can be better represented by the use of heavy-tail distributions, s...

A numerical transfer matrix method (TMM) is applied to investigate hybrid surface plasmon polaritons (HySPPs) waveguide structure, which consists of a high permittivity dielectric fiber separated from a metal surface with a low permittivity dielectric gap. The results obtained from the TMM agree well with those from the finite element method but with a faster calculation speed. As a demonstrati...