lots of viruses, in particular rna viruses, have high mutation rates and relatively short generation times. particle stability during infection in nature or in laboratory triggers the evolutionary event toward different mechanisms such as genome segmentation, point mutation and  recombination. the frequency of mutant genomes increase and modify  the previous distribution, which, consequently, l...

Abstract The development of approaches for predictive calculation hybridization properties various nucleic acid (NA) derivatives is the basis rational design NA-based constructs. Modern advances in computer modeling methods provide feasibility these calculations. We have analyzed possibility calculating energy DNA/RNA and RNA/RNA duplex formation using representative sets complexes (65 75 compl...

Background and Aims: Hepatitis C virus is one of the viral infections transmitted by blood transfusion. Patients with thalassaemia frequently need blood transfusion and are in danger of HCV infection. In most cases of infection (85%) the virus evades the immune system and establishes a chronic infection that may lead to cirrhosis and liver carcinoma. Liver is the main site of HCV replication ...

Magnetic nanoparticles separation technology is a method for quick and easy extraction biomolecules such as proteins, DNA and RNA. The present work describes total RNA isolation procedure from transformed rose petals in our laboratory using magnetic nanoparticles as a solid phase absorbant. Petals are the main sources of secondary metabolites, i.e. carotenoids, anthocyanins, flavonoids and phen...

background: breast cancer is the most common diagnosed cancer among women in the world. snail1 plays a role in the development of the invasive phenotypes of cancer, neural cell differentiation, cell division and apoptosis in tumor cells. traces of snail1 in metastasis of breast cancer to bone are observed. the aim of this study was to investigate the effect of specific snail1 sirnas on the prol...

introduction: although extensive research has been conducted on lung cancer markers, a singular clinically applicable marker has not been found yet. the objective of this study was to evaluate the sensitivity and the specificity of carcinoembryonic antigen (cea) mrna and lung-specific x protein (lunx) mrna biomarkers in peripheral blood to detect lung cancer individually and simultaneously. met...