The efficacy of iron(II) sulfide (FeS)-based autotrophic denitrification in simultaneous nitrogen and phosphorus removal from wastewater was studied with batch experiments. It was efficient at a wide pH range of 5-9, and temperature range of 10-40 °C. The concentrations of NH₄⁺-N, Mg²⁺ and HCO₃⁻ in the wastewater should be kept over 7.8, 0.24 and 30 mg L⁻¹ for efficient nitrate (NO₃⁻-N) reducti...