If gilts are known to be cyclic, the options to control estrus are breed-and-abort or feeding allyl trenbolone (Regumate®). For breed and abort, terminate pregnancy 25–30 days postbreeding with two injections of prostaglandin F2a (PGF2a), 6–8 hours apart. Gilts should return to estrus within 4–6 days with normal fertility. When feeding allyl trenbolone, you must ensure individual feeding so tha...

MicroRNAs (miRNAs) are negative modulators of gene expression that fine-tune numerous biological processes. miRNA loss-of-function rarely results in highly penetrant phenotypes, but rather, influences cellular responses to physiologic and pathophysiologic stresses. Here, we have reported that a single member of the evolutionarily conserved miR-7 family, miR-7a2, is essential for normal pituitar...

Lewis, D. A., M. P. Bracamonte, K. S. Rud, and V. M. Miller. Selected Contribution: Effects of sex and ovariectomy on responses to platelets in porcine femoral veins. J Appl Physiol 91: 2823–2830, 2001.—Estrogen replacement increases risk of venous thrombosis. In this study, we determined responses in vitro to platelets and platelet products in veins from adult male and intact and ovariectomize...

F2-Isoprostanes (IsoPs) are isomers of prostaglandin F2a formed from the nonenzymatic free radicalcatalyzed peroxidation of arachidonic acid. Since discovery of these molecules by Morrow and Roberts in 1990, F2-IsoPs have been shown to be excellent biomarkers as well as potent mediators of oxidative stress in vivo in humans. Isofurans (IsoFs) are also oxidation products generated from the nonen...

The Human Immunodeficiency Virus type 1 (HIV-1) accessory protein Nef interacts with a multitude of cellular proteins, manipulating the host membrane trafficking machinery to evade immune surveillance. Nef interactions have been analyzed using various in vitro assays, co-immunoprecipitation studies, and more recently mass spectrometry. However, these methods do not evaluate Nef interactions in ...

The potential roles of 2 reproductive hormones, prolactin and prostaglandin, in the regulation of nematode growth, fecundity, and survival in lambs were examined in 2 experiments. In the first experiment, prolactin (25 ILJ/lamb) was administered to 3 groups of lambs at 1-wk intervals during each of the first 3 wk of patency of Haemonchus contortus infections, respectively. Fecundity (eggs/femal...