Poly(lactic acid) (PLA) is the most used biopolymer for food packaging applications. Several strategies have been made to improve PLA properties for extending its applications in the packaging field. Melt blending approaches are gaining considerable interest since they are easy, cost-effective and readily available processing technologies at the industrial level. With a similar melting temperat...

Polyhydroxybutyrate (PHB) is a common carbon storage polymer among heterotrophic bacteria. It is also accumulated in some photoautotrophic cyanobacteria; however, the knowledge of how PHB accumulation is regulated in this group is limited. PHB synthesis in Synechocystis sp. PCC 6803 is initiated once macronutrients like phosphorus or nitrogen are limiting. We have previously reported a mutation...

Polymerase is a key enzyme involved in the biosynthesis of polyhydroxybutyrate (PHB), a well-known bacterial biodegradable polyester. In this study, we have established an in vivo assay system to analyze mutational effects of Ralstonia eutropha polymerase (termed PhbCRe) on the level of PHB accumulation in recombinant strains of Escherichia coli. This in vitro evolution system consists of polym...

Prohibitin (PHB), a major mitochondrial membrane protein, has been shown earlier in our laboratoryto regulate sperm motility via an alteration in mitochondrial membrane potential (MMP) in infertile men with poor sperm quality. To test if PHB expression is associated with sperm mitochondrial superoxide (mROS) levels, here we examined sperm mROS levels, high MMP and lipid peroxidation in infertil...

The effect of different growth conditions on the glycogen and poly-/I-hydroxybutyrate (PHB) content of the cyanobacterium Spirulina maxima is described. Under photoautotrophic growth conditions without any nutrient limitation, S. maxima exhibited a glycogen content of between 7-1 and 10.7% of cell dry wt, whereas PHB was undetectable. When S. maxima was grown under mixotrophic conditions in the...

Polyhydroxybutyrate molecules produced by recombinant Escherichia coli were evaluated in terms of their size in solution to develop and test a hypothesis concerning the regulation of the molecular weight of PHB during in vivo polymer production. Two recombinant strains of E. coli were used to produce material for this study: DH5a/pAeT41, containing the native operon from Alcaligenes eutrophus e...

BACKGROUND Vibrio pathogens are causative agents of mid-culture outbreaks, and early mortality syndrome and secondary aetiology of most dreadful viral outbreaks in shrimp aquaculture. Among the pathogenic vibrios group, Vibrio alginolyticus and V. harveyi are considered as the most significant ones in the grow-out ponds of giant black tiger shrimp Penaeus monodon in India. Use of antibiotics wa...

Direct polyhydroxybutyrate (PHB) production from starch was for the first time achieved using engineered Corynebacterium glutamicum expressing PHB biosynthetic genes and displaying α-amylase on its cell surface. The engineered strain accumulated 6.4 wt% PHB from starch which was higher than that obtained from glucose (4.9 wt%).