EDTA-derivatized deoxythymidine (dT-EDTA), incorporated into DNA and complexed to Fe2+ in the presence of dithiothreitol, is a widely used reagent for sequence-specific cleavage of duplex DNA. Using HPLC/electrospray mass spectrometry, we show that cleavage is specific to Fe2+, and no cleavage occurs when DNA-EDTA is complexed to other metal ions such as Ca2+, Mn2+, and Fe3+ even after many day...

EDTA: THE CHELATING AGENT UNDER ENVIRONMENTAL SCRUTINY. The chelating agent EDTA (ethylenediaminetetraacetic acid) is a compound of massive use world wide with household and industrial applications, being one of the anthropogenic compounds with highest concentrations in inland European waters. In this review, the applications of EDTA and its behavior once it has been released into the environme...

background: to investigate the effect of cana2-edta and experimental diabetes (iddm) on zinc and carbohydrate metabolism and the activities of got, gpt and alkaline phosphatase. methods: forty male normal albino (wistar) rats of 8 weeks of age were fed with a basal diet. twenty rats were then intraperitoneally injected with alloxan to induce diabetes. then after one week ten rats from each grou...

Objective: Photon-induced photoacoustic streaming (PIPS) with pineapple peel extract (PPE) and ethylenediaminetetraacetic acid (EDTA) as a final endodontic irrigant on the push-out bond strength (PBS) of root filling material to conventional irrigation NaOCl EDTA.
 Methods: An in vitro study at Dar Al Uloom University was conducted over three months. Root canal preparation performed. Based...

The lysis of certain gram-negative bacteria by ethylenediaminetetraacetic acid (EDTA) and lysozyme was first reported by Repaske (7, 8). Lysis by EDTA alone was reported for Pseudomonas aeruginosa (3, 8), Vibrio succinogenes (9), and Alkaligenes faecalis (3). The mechanism for lysis by EDTA (2) in conjunction with lysozyme has been attributed to the interruption of the integrity of the cell sur...

Aim: This study was designed to investigate the effect of different final irrigation protocols on apical sealing ability bioceramic and epoxy resin-based sealers.
 Materials methods: Thirty human single-rooted mandibular premolars were instrumented using ProTaper Next rotary files. Teeth randomly divided into three groups according regimen; Group I: 5 ml 0.2% CNPs/3 min, II: 2.5 CNPs/1.5 m...

Colorimetry of iron in serum with Ferrozine (as used in the Technicon SMAC) or with bathophenanthroline (as used in the Du Pont aca) is influenced by EDTA, in contrast to such measurements with atomic absorption spectroscopy. Therefore EDTA contamination should be avoided with these colorimetric methods. If, however, contamination with EDTA is suspected, addition of zinc sulfate to serum or to ...

electrokinetic remediation is one of the most promising in situ or ex situ processes for decontamination of heavy metals, anions and polar organic compositions from the soil. in this study, we investigated the efficacy of this method in removing mercury from clayey soil (with kaolin to sand weight ratio of 2:1) and from gold mine tailing dam sediment and determined optimum removal conditions. t...