Abstract The use of exogenous maltogenic ?-amylases or maltotetraogenic amylases bacterial origin is common in wheat bread production, mainly as antistaling agents to retard crumb firming. To study the impact ?-amylase and amylase on straight dough bread, we performed a discovery-driven proteomics approach with commercial enzyme preparations identified P22963 from Pelomonas saccharophila P19531...

α-amylases are ubiquitous enzymes belonging to the glycosyl hydrolase (GH13) family, whose members share a high degree of sequence identity, even between distant organisms. To understand determinants catalytic activity throughout evolution, and investigate use homologous as model for human one, we compared salivary α-amylase, Aspergillus oryzae α-amylase pancreatic porcine using combination in ...

Halophiles are perceived as an excellent source of novel enzymes possessing inherent ability to function under saline and hypersaline environment conditions. The article covers and puts in perspective the structural and biocatalytic features of α-amylases from halophilic sources. The choice of α-amylase as the target enzyme is based on the fact that this is among the largest selling enzymes. Ol...

A monoclonalantibody(66C7) was preparedthat specifically binds human salivary amylase (EC 3.2.1.1); it cross reacts with human pancreatic amylase by less than 1%. Two procedures are described for determination of isoamylases in human serum with this antibody: an enzyme immunoassay for determining amylase of salivary origin, and a routine method in which this amylase is immunoprecipitated and th...

The levels of starch, soluble sugars, protein, and enzymes involved in starch metabolism-alpha-amylase, beta-amylase, phosphorylase, Q-enzyme, R-enzyme, and starch synthetase -were assayed in dehulled developing rice grains (Oryzasativa L., variety IR8). Phosphorylase, Q-enzyme, and R-enzyme had peak activities 10 days after flowering, whereas alpha- and beta-amylases had maximal activities 14 ...