background hepatitis c virus (hcv) infection has been explained as a disease that sometimes present with rheumatic manifestations indistinguishable from rheumatoid arthritis. this study has been performed to evaluate the frequency of hepatitis c virus infection in a group of patients with rheumatoid arthritis. patients and methods in this study, during one year, serum samples collected from two...

objectives: the presence of e.coli in fish intended for human consumption may constitute a potential danger, not only in causing disease, but also because of the possible transfer of antibiotic resistance from aquatic bacteria to those infecting humans. the objective of this study was to develop an improved pcr method based on species – specific 16 s rrna gene primers (fes, res) for detection o...

identification  of clinically  significant  nocardia  species  is essential  for the definitive diagnosis, predict antimicrobial susceptibility, epidemiological purposes,  and  for  an  effective  treatment.   conventional   identification   of nocardia species in routine medical laboratories which is based on phenotypic (cellular morphology, colonial characteristics), biochemical and enzymatic...

rna extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. although many rna extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. many studies have shown that trizol is an applicable material for the rna extracti...

given the importance of transcriptome analysis in various biological studies and considering thevast amount of whole transcriptome sequencing data, it seems necessary to develop analgorithm to assemble transcriptome data. in this study we propose an algorithm fortranscriptome assembly in the absence of a reference genome. first, the contiguous sequencesare generated using de bruijn graph with d...

Background and Aims: Hepatitis C virus is one of the viral infections which is mainly transmitted by blood transfusion. Patients with thalassaemia frequently need blood transfusion and are in danger of HCV infection. In most cases of infection (85%) the virus evades the immune system and establishes a chronic infection that may lead to cirrhosis and liver carcinoma. Liver is the main site of HC...

Background:In the recent decade, the reverse genetics method has been broadly used for rescue of negative-stranded RNA viruses from cDNA or viral minigenomes. This technique has been applied to study different steps in virus replication and virus-host interactions. Reverse genetics could also be implemented for design of new vaccines. The T7 RNA polymerase activity as well as virus (nucleocapsi...