AIMS To develop a rapid multiplex PCR method for simultaneous detection of five major foodborne pathogens (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli O157:H7, Salmonella Enteritidis and Shigella flexneri, respectively). METHODS AND RESULTS Amplification by PCR was optimized to obtain high efficiency. Sensitivity and specificity assays were investigated by testing differen...

a multiplex pcr-based assay was developed for detection of salmonella typhi and identification of other salmonella serotypes. three primer-sets were selected from different genomic sequences, malo2-f/malo2-ra primers from invasion gene, parat-s/parat-as as well as tyv-s/tyv-as primers from o-antigen gene cluster of the genus salmonella. this method differentiated salmonella spp., based on size ...

background: infections caused by pseudomonas aeruginosa or acinetobacter baumannii are of greatest concern for hospitalized patients, particularly those in intensive care units (icus). the aims of this study were to investigate the prevalence of integrons and biofilm formation among p. aeruginosa and a. baumannii isolates collected from icu and non-icu inpatients. materials and methods: a total...

for identification of individuals and parentage control performed by cattle breeders in the czech republic, a novel finnish bovine genotypes™ panel 3.1was amplified by means of one multiplex polymerase chain reaction. bovine panel encompasses all the 12 str loci recommended by the international society for animal genetics (isag) for routine use in parentage testing and identification, including...

objective: bacillus cereus (b. cereus) is a gram-positive, spore-forming bacteria widely distributed in the environment. this bacteria is an opportunistic human pathogen that can cause diarrheal and emetic types of food poisoning. the diarrhea type of food poisoning can be caused by hemolysin bl (hbl), non-hemolytic (nhe), and cytotoxin k enterotoxins. rice is commonly contaminated with b. cere...

background and objectives:hbv and htlv-i are life threatening infectious agents in patients who receive blood and blood products. although serological methods have been proved to be useful, detection of these viruses has remained a challengingissue due to the many obstacles. by the advent of nucleic acid testing methods, especially in multiplex format, more precise detection is possible.the obj...

bovine brucellosis is a zoonotic disease distributed worldwide and characterized by abortion and reducedfertility in cows. since brucellosis eradication programme in iran uses vaccination, test, slaughter andquarantine as control measures, it is essential to distinguish vaccine strains from strains that cause infectionsamong vaccinated cattle herds. we developed and evaluated a multiplex polyme...

Existing reactions for the multiplex PCR amplification of exons in the dystrophin gene have been modified to produce two multiplex reactions which separately cover the 5' and 3' major deletion 'hotspots' in the gene, and together detect approximately 98% of all deletions detectable by Southern cDNA hybridisation. A comparative study of 148 patients showed mistypings in both the cDNA hybridisati...