نتایج جستجو برای: yac clone
تعداد نتایج: 39207 فیلتر نتایج به سال:
Juvenile Open Angle Glaucoma (GLC1A) is an autosomal optic neuropathy that has been localized previously to chromosome 1q. Here we report the fine mapping of the disease region using YACs and a high density of polymorphic microsatellite markers. This study utilized two large JOAG pedigrees genotyped at 36 loci from chromosome 1q21-q31 to refine the GLC1A locus to a approximately 3-cM region fla...
Autosomal dominant familial spastic paraplegia (AD-FSP) is a genetically heterogeneous neurodegenerative disorder characterized by progressive spasticity of the lower limbs. Three loci on chromosome 14q (SPG3), 2p (SPG4), and 15q (SPG6) were shown to be responsible for AD-FSP. Analysis of recombination events in three SPG3-linked families allowed us to narrow the critical interval from 9 to 5 c...
A new hybrid experiment has been started by ASγ experiment at Tibet, China, since August 2011, which consists of a low threshold burst-detector-grid (YAC-II, Yangbajing Air shower Core array), the Tibet air-shower array (Tibet-III) and a large underground water Cherenkov muon detector (MD). In this paper, the capability of the measurement of the chemical components (proton, helium and iron) wit...
The short arm of human chromosome 5 contains approximately 48 Mb of DNA and comprises 1.5% of the genome. We have constructed a mega-YAC/ STS map of this region that includes 436 YACs anchored by 216 STSs. By combining and integrating our map with the 5p maps of other groups using the same recombinant DNA library, a comprehensive map was constructed that includes 552 YACs and 504 markers. The Y...
Representational difference analysis (RDA), a subtractive hybridization method that enriches differences between complex genomes, can be used to isolate fragments deleted in tumor genomes. Usually, most of the clones obtained by this approach result from polymorphic fragments. Therefore, identification of homozygously deleted fragments, which can indicate the presence of tumor suppressor loci, ...
A novel strategy for separation of co-cloned YACs was developed. For this, yeast cells were grown under non-selective conditions to allow the mitotic loss of multiple YACs. Yeast colonies of different size appear on 'drop-out' selection plates with small clones consistently containing a single-copy YAC. Different auxotrophic marker genes can be used to separate co-cloned YACs or reduce their co...
poplar trees are becoming extremely important as fast growing species due to the increasing need for wood and decreasing productivity of some lands in iran. the present study aimed to find the effect of canopy of poplar clones as one of the most important effective factors on soil properties compared to the distances inter-row trees. five poplar clones and species included: populus euramericana...
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