Methods based on DNA reassociation in solution with the subsequent PCR amplification of certain hybrid molecules, such as coincidence cloning and subtractive hybridization, all suffer from a common imperfection: cross-hybridization between various types of paralogous repetitive DNA fragments. Although the situation can be slightly improved by the addition of repeat-specific competitor DNA into ...

Non-isolated congenital diaphragmatic hernia (CDH+) is a severe birth defect that is often caused by de novo chromosomal anomalies. In this report, we use genome-wide oligonucleotide-based array comparative genome hybridization (aCGH) followed by rapid real-time quantitative PCR analysis to identify, confirm and map chromosomal anomalies in a cohort of 26 CDH+ patients. One hundred and five put...

Genome-wide copy number profiles were characterized in 41 primary bladder tumors using array-based comparative genomic hybridization (array CGH). In addition to previously identified alterations in large chromosomal regions, alterations were identified in many small genomic regions, some with high-level amplifications or homozygous deletions. High-level amplifications were detected for 192 geno...

A novel fluorescent nanosensor has been developed by combining super fluorescence quenching ability of graphene oxide and hybridization chain reaction amplification, which enables highly sensitive detection of base excision repair enzyme activity with a wide dynamic range from 0.0001 to 100 U mL(-1) and a detection limit of 0.00006 U mL(-1).

background and aims: viroids are smallest known plant pathogens and cause several economically significant diseases. until recently, viroid detection relied mainly on biological tests and indexing. today various diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (rt-pcr) are being used for detection and diagno...

in order to produce new hybrids and evaluate the general and specific combining ability of sunflower inbred lines and restorers, four inbred lines and six restorers were selected in 2004. twenty-four f1 hybrids were derived in 2004 and evaluated on a rcbd design with three replications in 2005. the results of variance analysis showed that treatments were significantly different for all traits e...

Inter-species hybridization has been recently recognized as potentially common in wild animals, but the extent to which it shapes modern genomes is still poorly understood. Distinguishing historical hybridization events from other processes leading to phylogenetic discordance among different markers requires a well-resolved species tree that considers all modes of inheritance and overcomes syst...

Hybridization is an evolutionary process that can generate diverse outcomes, such as reinforcing species boundaries, generating new species, or facilitating the introgression of locally-adapted alleles into genomic backgrounds. Liolaemus a highly clade South American lizards with ~260 and many ten are described each year. Previous studies have detected gene flow among using phylogenetic network...

Abstract Hybridization is of key relevance for conservation wild species, and yet it still one the most controversial issues in conservation. Identifying historical contemporary factors promoting formation maintenance hybrids crucial management endangered as well characterizing environmental patterns habitat use among parental species their hybrids. Crocodiles are a priority group since all 23 ...