Case 1 GB is a 4 month old male currently living with his aunt. His mother is deceased and nothing is known about the mother’s health or the birth history. Over the last 4 weeks, GB developed worsening watery non-bloody diarrhea and felt warm to his caregivers. Over the last week his oral intake decreased significantly; he is being fed formula and phala (soft maize porridge). On examination, GB...

Bourdieu’s intermittent allusions to Spinoza’s conatus disclose the weaknesses of his concept habitus. A thorough inspection involvement with Spinozist legacy reveals a long-lasting inconsistency, for he expects that will assist him in both 1) grounding habitus and solving uncertainties surround this notion by endorsing strong conatus, impervious resistances it eventually encounter; 2) re-insta...

There is evidence that alcohol consumption among those in middle-class occupations consistently exceeds safe levels, yet there has been little research into why this occurs. This article explores the meanings associated with alcohol use among professional, managerial and clerical workers. Qualitative data were collected from five focus groups of male and female employees aged 21-55 (N =49: 32 m...

This paper explores the contribution and the influence of system dynamics in the development of management and organisation theory. It begins with a brief discussion of the contribution of computer modelling and simulation in theory development for the above areas. It then discusses the practice of management theory development from the sociology of science perspective using Bourdieu’s concepts...

Both the amino-terminal and carboxyl-terminal domains of IGF binding protein (IGFBP)-3 are believed to contribute to high-affinity IGF binding. To investigate cooperativity in IGF binding by these domains, we expressed IGFBP-3 fragments 1-88 (NBP-3) and 185-264 (CBP-3) as FLAG and hexahistidine-tagged fusion proteins, respectively. IGF-I and IGF-II bound to NBP-3 poorly and to CBP-3 with modera...

The major light-harvesting chlorophyll a/b-binding protein (Lhcb1,2) of photosystem II is inserted into the thylakoid via the signal recognition particle dependent pathway. However, the mechanism by which the protein enters the membrane is at this time unknown. In order to define some topographical restrictions for this process, we constructed several recombinant derivatives of Lhcb1 carrying h...

Hairy root culture is a potential alternative to conventional mammalian cell culture to produce recombinant proteins due to its ease in protein recovery, low costs and absence of potentially human pathogenic contaminants. The current study focussed to develop a new platform of a hairy root culture system from Nicotiana tabacum for the production of recombinant human EPO (rhEPO), which is regula...

data collected confirmed the RNA-Seq results. ChIP-Seq methods are being optimized to identify the direct targets of OpaR. Select targets were confirmed direct targets via in vitro electrophoretic mobility shift assays (EMSA) with purified hexahistidine tagged OpaR. A previously published position specific weighted matrix (PSWM) will be utilized to assist in identification of possible OpaR bind...