The chi (chi) and psi (psi) subunits of Escherichia coli DNA polymerase III form a heterodimer that is associated with the ATP-dependent clamp-loader machinery. In E. coli, the chi:psi heterodimer serves as a bridge between the clamp-loader complex and the single-stranded DNA-binding protein. We determined the crystal structure of the chi:psi heterodimer at 2.1 A resolution. Although neither ch...

Current proposals for load-time transformation of Java classes are either dependent on the use of a specific class loader or dependent on a specific JVM implementation. This is not due to an inadequacy of the Java platform but to the wrong choice of the level at which to hook into the Java Class Loader Architecture. JMangler follows a novel approach that ensures both class loader and JVM indepe...

This paper describes the module loader concept. We successfully used this for the development of system software for our mobile robots. The module loader allows for organizing the software in modules. These can be loaded and removed at runtime. We describe how this is done. Additionally we give some examples on how to use the concept. Exemplified on our robot platforms we describe how to design...

As embedded systems continue to evolve, Java becomes an attractive alternative to traditional languages for embedded hardware platforms. However, there are a number of obstacles for Java to hurdle in order to fulfill all the requirements of any real-time embedded systems. This paper presents an architectural view of a memory design for the embedded Java systems. By introducing a new static load...

The Structural Maintenance of Chromosome (SMC) complex, termed cohesin, is essential for sister chromatid cohesion. Cohesin is also important for chromosome condensation, DNA repair, and gene expression. Cohesin is comprised of Scc3, Mcd1, Smc1, and Smc3. Scc3 also binds Pds5 and Wpl1, cohesin-associated proteins that regulate cohesin function, and to the Scc2/4 cohesin loader. We mutagenized S...

Existing designs of screw working bodies transport and processing machines cannot always provide the required performance avoid overloading, which causes deformation damage to parts mechanisms. Thus, it is necessary establish new efficient that would ensure execution technological process. The purpose study was substantiate explore design methodology for calculating main structural-power parame...

Escherichia coli γ complex clamp loader functions to load the β sliding clamp onto sites of DNA replication and repair. The clamp loader uses the energy of ATP binding and hydrolysis to drive conformational changes allowing for β binding and opening, DNA binding, and then release of the β·DNA complex. Although much work has been done studying the sliding clamp and clamp loader mechanism, kineti...

Ring-shaped sliding clamps and clamp loader ATPases are essential factors for rapid and accurate DNA replication. The clamp ring is opened and resealed at the primer-template junctions by the ATP-fueled clamp loader function. The processivity of the DNA polymerase is conferred by its attachment to the clamp loaded onto the DNA. In eukarya and archaea, the replication factor C (RFC) and the prol...

MOTIVATION Biological data come in very different shapes. Databanks are maintained and used by distinct organizations. Text is the de facto Standard exchange format. The SRS system can integrate heterogeneous textual databanks but it was lacking a way to structure the extracted data. RESULTS This paper presents a CORBA interface to the SRS system which manages databanks in a flat file format....

It has been proved by Loader [2] that Statman-Plotkin conjecture (see [5] and [4]) fails. The Loader proof was done by encoding the word problem in the full type hierarchy based on the domain with 7 elements. Later Loader refined the result by establishing that the problem is undecidable for every finite domain with 3 ground elements [3]. The remaining non trivial case with 2 ground elements ha...