Aim: To know the magnitude of influence by Egg Drop Syndrome – 1976 (EDS –'76) virus infection in causing drop in egg production in and around Namakkal. Materials and Methods: A total of 150 cloacal swabs and 15 pouch shell glands (uteri) homogenates from 15 poultry farms in and around Namakkal area were used for virus isolation. Three numbers of 10 –dayold embryonated duck eggs were used for t...

THE importance of infectious hepatitis as a world-wide problem has been highlighted in detail during the presentations made at this symposium. It is clear that as of today (May I 8, I968) there is no published report confirming the isolation of infectious hepatitis virus in a tissue-culture system or animal host. Experience with poliomyelitis, measles, and rubella has revealed that the time int...

Influenza virus was recovered from aqueous suspensions by means of membrane filters. Separation of virus from bacterial mixtures was achieved and virus was recovered free from bacteria. Technics developed for this application of the use of membrane filters were presented. These technics were extended to the successful isolation of Asian influenza virus from clinical specimens made available for...

We report the isolation of dengue virus type 2 from a dengue patient returning to Japan from Nepal in October, 2004. This is the first isolate of dengue virus in Nepal. According to nucleotide homology, the virus was closest to a dengue virus type 2 isolate from India.

Immunoglobulin G antibody to human cytomegalovirus (CMV)-specific early antigens (EA-Ab) was determined by the immunoperoxidase antibody technique in several cases of congenital, primary, and reactivated CMV infections. Mothers of congenitally infected infants and a group of leukemic children and pregnant women were also studied. In 11 cases of congenital infection, CMV EA-Ab was always associa...

To identify tickborne viruses circulating in Kenya and the surrounding region, we conducted surveillance at abattoirs in Nairobi, Kenya. Species of ticks collected included Rhipicephalus pulchellus (56%), Amblyomma gemma (14%), R. appendiculatus (8%), A. variegatum (6%), and others. A total of 56 virus isolates were obtained, 26 from A. gemma, 17 from R. pulchellus, 6 from A. variegatum, and 7 ...

Bluetongue virus (BTV) infects sheep, cattle and other ruminants and is transmitted by Culicoides spp. of biting midges. Virus is typically isolated and characterized by infection of susceptible vertebrate cells that undergo detectable and measurable cytopathic effects. Cell lines derived from C. sonorensis may be useful for virus isolation and studies to better understand BTV replication in th...

Trachea, lung, and conjunctive samples from 51 commercial layer farms from Bastos region, São Paulo, Brazil, were submitted to nestedPCR and virus isolation in SPF chicken embryos for ILT diagnosis. This region experienced an outbreak characterized by respiratory signs, decrease in egg production and increased mortality. Out of the 51 tested field samples, 23 were positive for ILT by nested-PCR...

We estimated the proportion of persons with pandemic (H1N1) 2009 who were shedding infectious virus at diagnosis and on day 8 of illness. In households with confirmed cases, nasopharyngeal swabs were collected on all members and tested by PCR and virus culture. Of 47 cases confirmed by PCR at &lt7 days of illness, virus culture was positive in 92% (11/12) of febrile and 63% (22/35) of afebrile ...