The O acetylation of peptidoglycan occurs specifically at the C-6 hydroxyl group of muramoyl residues. Using a combination of high-performance liquid chromatography-based organic acid analysis and carbohydrate analysis by high-pH anion-exchange chromatography, we determined that strains of Entercoccus durans, E. faecalis, E. faecium, and E. hirae produce O-acetylated peptidoglycan. The levels o...

Flow cytometry (FC) has been introduced to characterize and to assess the physiological states of microorganisms in conjunction with the classical plate-counting method. To show the applicability of the technique, in particular for the development of kinetic models, pure culture fermentation experiments were followed over time, using both prokaryotic (Lactobacillus hilgardii) and eukaryotic (Sa...

This special issue of the International Journal of Microbiology focuses on global health. More specifically, our aim is to establish whether there is a role for microbial ecology in global health. " Global health " is often defined as the " health of populations " a problem that transcends geographic boundaries and exists at the intersection of demography, economics, epidemiology, political eco...

BACKGROUND Loss of viability is one of the most important problems during starter culture production. Previous research has mostly focused on the production process of bacterial starters, but there are few studies about cellular protein deterioration causing cell defectiveness during storage. In the present study, we investigated the influence of storage temperature (-21, 4, 35°C) on the cellul...

Many species of bacteria, under conditions stress or nutrient limitation, enter a state dormancy referred to as viable but non-culturable (VBNC). VBNC bacteria persist in the environment, are difficult detect and identify by many standard laboratory methods can be altered their susceptibility antibiotics. Entry into is often accompanied morphological changes; however, mechanisms underlying this...

The elaboration of guidelines for the industry to establish minimum concentration prevent cross-contamination during washing practices based on operational limits is core recommended criteria use sanitizers. Several studies have evidenced that sanitizers reduced levels foodborne pathogens. However, they might lead progress into a viable but non-culturable (VBNC) state cells. This evidence has r...