Crystal structures of angiotensin-converting enzyme (ACE) complexed with three inhibitors (lisinopril, captopril, enalapril) provided experimental data for testing the validity of a prior active site model predicting the bound conformation of the inhibitors. The ACE active site model - predicted over 18 years ago using a series of potent ACE inhibitors of diverse chemical structure - was recrea...

T he reformatting and changes that were made to the Asthma Control Questionnaire (ACQ) [1] for the Post-cold Asthma Control and Exacerbation (PAX) study [2] raise some important concerns about modifying validated questionnaires. Just like mechanical and electrical measurement instruments, validated questionnaires are precision measurement instruments. The only difference being that they measure...

Introduction: Sleep spindles, the hallmark of sleep synchronization in stage 2 non-REM sleep, are rhythmic and monomorphic waves, between 10 and 14 Hz, with a maximum amplitude in the vertex (Cz), with bilateral irradiation to central regions (C3 and C4). Objective: In this article we present an overview of sleep spindles ranging from well established concepts of morphology, generation mechanis...

For a long time, the exploration of emotions focused on facial expression, and vocal expression of emotion has only recently received interest. However, no validated battery of emotional vocal expressions has been published and made available to the researchers’ community. This paper aims at validating and proposing such material. 20 actors (10 men) recorded sounds (words and interjections) exp...

The measurement of the effect of new chemical entities on human cytochrome P450 marker activities using in vitro experimentation represents an important experimental approach in drug development. In vitro drug interaction data can be used in guiding the design of clinical drug interaction studies, or, when no effect is observed in vitro, the data can be used in place of an in vivo study to clai...

CRISPRz (http://research.nhgri.nih.gov/CRISPRz/) is a database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish. Programmable RNA-guided CRISPR/Cas9 has recently emerged as a simple and efficient genome editing method in various cell types and organisms, including zebrafish. Because the technique is so easy and efficient in zebrafish, the most valuable asset ...

Based on work originating in the early 1970s, a number of recent global optimization algorithms have relied on replacing an original nonconvex nonlinear program by convex or linear relaxations. Such linear relaxations can be generated automatically through an automatic differentiation process. This process decomposes the objective and constraints (if any) into convex and nonconvex unary and bin...

Some new methods will be presented for computing verified inclusions of the solution of large linear systems. The matrix of the linear system is typically of band or sparse structure. There are no prerequisites to the matrix such as being M-matrix, symmetric, positive definite or diagonally dominant. For general band matrices of lower, upper bandwidth p, q of dimension n the computing time is l...

OBJECTIVE To evaluate the reproducibility of a semi-quantitative FFQ used in the Seguimiento Universidad de Navarra (SUN) project. DESIGN The data that were analysed were collected from an FFQ answered twice by a 326-participant subsample of the SUN project (115 men, 35.3 %; 211 women, 64.7 %), with either less than 1 year or more than 1 year between responses. The questionnaire included 136 ...

Many practical problems require nonparametric estimates of regression functions, and local polynomial regression has emerged as a leading approach. In applied settings practitioners often adopt either the local constant or local linear variants, or choose the order of the local polynomial to be slightly greater than the order of the maximum derivative estimate required. But such ad hoc determin...