Packed-column supercritical fluid chromatography (pSFC) with detection using atmospheric pressure chemical ionisation (APCI) mass spectrometry (MS) provides a versatile method for the detection and quantification of 20 polymer additives, including common antioxidants, light stabilisers and slip agents. Using MS with APCI in both positive and negative ion modes both molecular mass data and infor...

Anthocyanins are bioactive compounds which, due to their free radical scavenging properties, can protect the human body against oxidative stress that cause many diseases, such as cancer, aging. The Tuskegee purple Cap Vert and sweet potato (Ipomoea batatas L.) varieties rich in anthocyanins. Indeed, total anthocyanin contents estimated at 0.211 0.122 mg/g of fresh plant material, respectively. ...

The most widely used ionization techniques in liquid chromatography-mass spectrometry (LC-MS) are electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). All three provide user friendly coupling of LC to MS. Achieving optimal LC-MS conditions is not always easy, however, owing to the complexity of ionization processes and t...

Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) techniques for liquid chromatography-tandem mass spectrometry (LC-MS/MS) determination of levonorgestrel were evaluated. In consideration of difference in ionization mechanism, the two ionization sources were compared in terms of LC conditions, MS parameters and performance of method. The sensitivity for detection...

The presence and identity of very long chain polyunsaturated fatty acids from three freshwater crustacean species, Bathynella natans, B. baicalensis and Baicalobathynella magna from Lake Baikal and caves of central Europe were determined by means of liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization (LC-MS with APCI). LC-MS with APCI enabled the identification...

An analytical methodology based on high-resolution high mass accuracy electrospray ionization (ESI) tandem MS assisted by Se-specific detection using inductively coupled plasma mass spectrometry (ICP MS) was developed for speciation of selenium (Se) in seeds of black mustard (Brassica nigra) grown on Se-rich soil. Size-exclusion LC-ICP MS allowed the determination of the Se distribution accordi...

A simple high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) method for screening and quantification of ricinine in feeds is established for use in feed quality control. Ricinine is extracted from the sample with methanol. The sample extract is directly used for screening with LC-MS and is cleaned up using solid-phase extraction for quantification w...

Zeaxanthin is a predominant xanthophyll in human eyes and may reduce the risk of cataracts and age-related macular degeneration. Spirulina is an algal food that contains a high concentration of zeaxanthin. In order to determine the zeaxanthin bioavailability of spirulina for dietary supplementation in humans, spirulina was grown in nutrient solution with ²H₂O for carotenoid labelling. Single se...

The modification of high-performance liquid chromatography parameters leads to a more effective oligonucleotide-A purification process. Using various experimental such as buffer, concentration, and pH, method for optimizing the an on reverse-phase C18 column was created. To purify oligonucleotide-A, High-Performance Liquid Chromatography (HPLC), Ultraviolet-Visible Spectrophotometry (UV-Vis), C...