A common rate-limiting step in many high throughput proteome scale analyses is the cloning of predicted open reading frames (ORFs) into technique-specific vectors. For example, methodologies for the detection of protein interactions such as Yeast-two-hybrid (Y2H) assay require validation using alternative methods as Protein Fragment Complementation Assays (PCA) or pulldown experiments. Various ...

background: the clinical diagnosis of meningitis is crucial, particularly in children. the early diagnosis and empiric an­tibi­otic treatments have led to a reduction in morbidity and mortality rates. pcr and the enzymatic digestion of 16srdna frag­ment which is produced by universal primers led up fast and sensitive determination. the purpose of this study was to investi­gate a rapid method fo...

Maturity-onset diabetes of the young (MODY) is a form of NIDDM characterized by an early age of onset and autosomal-dominant inheritance (1). So far, three MODY-causing genes have been identified; namely, the hepatocyte nuclear factor4a (HNF-4a)/M0DYl on chromosome 20q, the glucokinase/M0DY2 on chromosome 7p, and the HNFla/M0DY3 on chromosome 12 q (2-4). The identification of the MODY genes has...

While metabarcoding of plant DNA from their environment is an exciting method that can supplement inventorying live species, the accuracy and specificity has yet to be fully assessed over complex continuous landscapes. In this work, we evaluate community profiles produced via soil by comparing them a morphological survey. We communities in 130 sites along ecological gradients (nutrients, succes...

randomly amplified polymorphic dna (rapd) markers were used to analyze genetic stability of the somatic embryogenesis-derived regenerants (r1-6) and mother plant in iranian date palm (phoenix dactylifera l.) cultivar khanizi. total genomic dna extracted from in vitro fresh leaves of regenerated plants and mother plant was amplified using 10-mer oligonucleotide fermantas primers. four primers of...

Several site-directed mutagenesis methods have been developed and are being currently used in research. All these methods apply three or four primers, including one or two bearing the appropriate mutation, and most use PCR to amplify the desired sequence and to incorporate the mutation (1–6). Although several developments have improved the robustness and reliability of the method, all of them r...