Urea synthesized commercially and formed naturally as a by-product of cellular metabolism is an important source of nitrogen (N) for primary producers in aquatic ecosystems. Although urea is usually present at ambient concentrations below 1 μM-N, it can contribute 50% or more of the total N used by planktonic communities. Urea may be produced intracellularly via purine catabolism and/or the ure...

Corynebacterium propinquum is a Gram-positive rod occasionally recovered from clinical infections which, according to 16S rRNA gene sequencing, is most closely related (>99% sequence similarity) to Corynebacterium pseudodiphtheriticum. The two species are very similar biochemically, commonly differentiated by a single test, the detection of urease, where strains of C. propinquum are described a...

Soybean (Glycine max [L.] Merrill) mutant aj6 carries a single recessive lesion, aj6, that eliminates ubiquitous urease activity in leaves and callus while retaining normal embryo-specific urease activity. Consistently, aj6/aj6 plants accumulated urea in leaves. In crosses of aj6/aj6 by urease mutants at the Eu1, Eu2, and Eu3 loci, F(1) individuals exhibited wild-type leaf urease activity, and ...

abstract background: streptococcus mutans in the oral cavities sable to produce mutacin (bacteriocin-like substances) with antibiotic properties. the aim of this study was to investigate the frequency and expression of genes encoding mutacins typei, ii, iii and iv and also two of 8 genes in a cluster encoding the putative bacteriocins, the designated bsm 283, bsm 299, bsm 423, bsm 1889c, bsm 18...

17 We report the first case of sepsis with enterocolitis that was caused by a 18 novel urease-positive Helicobacter species in a young man. The isolates were 19 characterized via 16S rRNA gene sequencing and their biochemical properties, and the 20 patient was successfully treated with short-term antimicrobial therapy; no recurrence 21

Helicobacter pylori is a human pathogen that infects over 50% of the population worldwide. It is the most important etiologic agent of gastroduodenal ulcers and malignancies. Helicobacter pylori urease enzyme is considered the main factor for the organism's colonization in the gastroduodenal mucosa. Hens immunized with the purified urease produce a highly specific anti-H. pylori urease immunogl...

Studies on enzyme inhibition remain a crucial area in drug discovery since these studies have led to the discoveries of new lead compounds useful in the treatment of several diseases. In this study, protocatechuic acid (PCA), an active compound from Hibiscus sabdariffa L. has been evaluated for its inhibitory properties against jack bean urease (JBU) as well as its possible toxic effect on huma...

Helicobacter hepaticus causes disease in the liver and lower intestinal tract of mice. It is strongly urease positive, although it does not live in an acidic environment. The H. hepaticus urease gene cluster was expressed in Escherichia coli with and without coexpression of the Helicobacter pylori nickel transporter NixA. As for H. pylori, it was difficult to obtain enzymatic activity from reco...

A rapid, miniaturized, urea broth test useful for detecting urease activity of yeasts was compared to Christensen urea agar. All urease-producing yeasts tested were positive on both media; however, 60% were reactive in the urea R broth within 30 min, and the remainder were reactive within 4 h. This urea multiwell test may be useful as a rapid screening method for detecting urease-producing yeas...

Stlmnltary The mechanism by which Helicobacter eylori, a noninvasive bacterium, initiates chronic antral gastritis in humans is unknown. We now show that H. Fflori rdeases products with chemotactic activity for monocytes and neutrophils. This chemotactic activity was inhibited by antisera to either H. ~lori whole bacteria or H. pylor/-derived urease. Moreover, surface proteins extracted from H....