Purpose: To develop a multiplex-urease PCR assay specific for ureA and ureB genes directly from gastric biopsy specimens and compare the results of multiplex-urease PCR assay with rapid urease test (RUT) and histopathology. Methodology: This study was conducted on 109 patients. A RUT was run; histopathological staining and multiplex urease PCR were applied to biopsy specimen to detect H. pylori...

In the preceding paper (l), hydroxyurea was shown to be a substrate for urease and to be associated with the development of a marked inhibition of the enzyme during t,he course of the reaction. The lack of significant inhibition during the hydrolysis of urea at equal concentrations suggested that the hydroxylamine formed during hydroxyurea hydrolysis was the responsible agent. The inhibitory ef...

Urea, a non-protein nitrogen for dairy cows, is rapidly hydrolyzed to ammonia by urease produced by ureolytic bacteria in the rumen, and the ammonia is used as nitrogen for rumen bacterial growth. However, there is limited knowledge with regard to the ureolytic bacteria community in the rumen. To explore the ruminal ureolytic bacterial community, urea, or acetohydroxamic acid (AHA, an inhibitor...

Four soybean meals (SBM) were prepared in a commercial solvent-extraction plant to give a much wider range in heat treatment than is usually found among commercially available SBM. The meals were designated in ascending order of heat treatment as Under, Normal, Over and Rumen Escape. The Normal meal was processed using standard operating conditions. The Under meal received less heat treatment b...

The kinetic characteristics of soil urease have attracted great attention, but little information was available on its kinetic and thermodynamic behaviors as affected by urease inhibitors and temperature. With black soil (Pachic Udic Mollisol) in Heilongjiang Province of Northeast China as a test soil, an incubation test was conducted to investigate the effect of urease inhibitors (hydroquinone...

Since the discovery of Helicobacter pylori, the urease activity of this bacterial pathogen has been identified as the key factor in infection and acid acclimation in the human stomach. Ureolytic activity plays a key role in the pathogenesis of several bacteria, and urease has also been described as an emerging pathogenic factor during fungal infection. However, urease produced by the oral bacte...

BACKGROUND Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs against urease B that neutralize the enzyme's activity. We mapped their epitopes ...

Urease is an important enzyme both in agriculture and medicine research. Strategies based on urease inhibition is critically considered as the first line treatment of infections caused by urease producing bacteria. Since, urease possess agro-chemical and medicinal importance, thus, it is necessary to search for the novel compounds capable of inhibiting this enzyme. Several computational methods...

SoilScience • Volume 175, Number 11, November 2010 Urease is one of the most thoroughly studied soil enzymes because it greatly influences the fate and performance of a widely used fertilizer (urea) (Bremner and Mulvaney, 1978; Ma et al., 1999; Tate, 2002). Urea was reported to be taken up by microorganisms, such as Corynebacterium glutamicum and Paxillus involutus (Jahns and Kaltwasser, 1989; ...