In underbalanced drilling (UBD), the bottom hole pressure is kept below pore pressure, causing pressure-dependent influx of reservoir gas into wellbore that makes system unstable at low drawdowns. this article, we propose a feedback controller which stabilizes system, represented by “reduced drift flux model (DFM),” around an arbitrary setpoint, while using only topside measurement and assuming...

Cables are usually bent into a U-shape to cross obstacles during installation: this includes the upward-bending mode (UBM) and downward-bending (DBM). An experimental study was conducted investigate combustion behavior of U-shaped cables with above bending forms different angles. The ignition point set in middle temperature distribution, flame spread rate (FSR), mass loss (MLR), dimensional cha...

Deubiquitinating enzymes (DUBs) are proteases that fulfill crucial roles in the ubiquitin (Ub) system, by deconjugation of Ub from its targets and disassembly of polyUb chains. The specificity of a DUB towards one of the polyUb chain linkages largely determines the ultimate signaling function. We present a novel set of diubiquitin FRET probes, comprising all seven isopeptide linkages, for the a...

The ubiquitin-like modifier FAT10 targets proteins for degradation by the proteasome and is activated by the E1 enzyme UBA6. In this study, we identify the UBA6-specific E2 enzyme (USE1) as an interaction partner of FAT10. Activated FAT10 can be transferred from UBA6 onto USE1 in vitro, and endogenous USE1 and FAT10 can be coimmunoprecipitated from intact cells. Small interfering RNA-mediated d...

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology Pharmacology (MP08)1 Sep 2021MP08-04 PHENOTYPIC CHARACTERIZATION OF A Mas1 RECEPTOR WILDTYPE AND KNOCKOUT ON BLADDER PAIN IN RATS Maia Terashvili, Bidyut Medda, Bhavana Talluri, Banani Banerjee, and Jyoti Sengupta TerashviliMaia Terashvili More articles by this author , MeddaBidyut Medda TalluriBhavana Talluri BanerjeeBanan...

We describe the genetically directed incorporation of aminooxy functionality into recombinant proteins by using a mutant Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNACUA pair. This allows the general production of nonhydrolysable ubiquitin conjugates of recombinant origin by bioorthogonal oxime ligation. This was exemplified by the preparation of nonhydrolysable versions of diubiquitin...