Complementary DNA was transcribed from turnip yellow mosaic virus RNA, using the method of Taylor et al. (1). The purified cDNA thus obtained sedimented between 2 and 4 S and was a mostly uniform transcript of template RNA. It hybridized with a sharp transition to homologous TYMV-RNA (Crt 1/2 = 2.7 x 10(-2)), but showed a low level of hybridization (less than 5%) to the RNAs of two other tymovi...

Turnip crinkle virus is one of several single-stranded RNA plant viruses associated with defective interfering (DI) RNAs. A complete cDNA copy of a 344-base DI RNA (DI RNA G) was cloned downstream from a T7 RNA polymerase promoter. Transcripts synthesized in vitro were infectious when inoculated with helper virus on turnip plants. Studies of the infectivity of DI transcripts containing deletion...

The coat protein of belladonna mottle virus (a tymovirus) was cleaved by trypsin and chymotrypsin, and the peptides were separated by high performance liquid chromatography using a combination of gel permeation, reverse phase, and ion pair chromatography. The peptides were sequenced manually using the 4-N, N-dimethylaminoazobenzene-4'-isothiocyanate/phenyl isothiocyanate double-coupling method....

Turnip mosaic virus (TuMV) is an economically important infecting a broad range of arable and vegetable crops many wild plant species. It also particular scientific interest as it has the broadest host any potyviruses, infects dicotyledonous monocotyledonous plants, transmitted by (>89) aphid species best adapted potyvirus to Arabidopsis thaliana. For these reasons been particularly well studie...

The 206-kDa protein of turnip yellow mosaic virus belongs to an expanding group of proteins containing a domain which includes the consensus nucleotide binding site GxxxxGKS/T. A portion of this protein (amino acids [aa] 916 to 1259) was expressed in Escherichia coli and purified by affinity chromatography to near homogeneity. In the absence of any other viral factors, it exhibited ATPase and G...

Using a polyethylene glycol-mediated uptake procedure, protoplasts from a range of Cruciferous species were infected with cauliflower mosaic virus; they include Moricandia arvensis, a plant not k n o w n to be susceptible. With this technique > 90~o of turnip protoplasts were infected, each producing an average of 104 to 105 viral genome equivalents. Filter hybridization and fluorescent antibod...