The formation of C'1 esterase from C'1, the first component of complement, may be brought about by the action of plasmin or trypsin upon C'1s, a subcomponent of C'1. These enzymes also decrease the esterolytic activity of C'1 esterase. The formation of C'1 esterase was demonstrated by measuring the appearance of an agent or agents with esterolytic properties and the capacity to inactivate C'2 a...

Sponge gourd (Luffa cylindricd) seeds contain several biologically active proteins. Recently, we found that the mature seeds contain three types of trypsin inhibitors having molecular masses of about 13 kDa (type I), 6.5 kDa (type II), and 3.2 kDa (type III). Furthermore, preliminary experiments suggested that the type III one might be a domain unit of these inhibitors. Here, we present the iso...

When factor VIII/von Willebrand factor (FVIII/vWF) is chromatographed on 4% agarose in 0.25 M CaCI2. the procoagulant activity partially separates from the void volume protein peak. If FVIII/vWF is reacted with thrombin prior to chromatography, both the magnitude and resolution of the proe.oagulant activity peak are greatly increased. These observations suggest that activated species of FVIII/v...

The structure of bovine pancreatic trypsin inhibitor has been solved in a new crystal form III. The crystals belong to space group P2(1)2(1)2 with a = 55.2 A, b = 38.2 A, c = 24.05 A. The structure was solved on the basis of co-ordinates of forms I and II of the inhibitor by molecular replacement, and the X-ray data extending to 1.7 A were used in a restrained least-squares refinement. The fina...

From among a wide variety of protein purification techniques affinity chromatography has proved to be particularly effective for separation of proteolytic enzymes and their inhibitors. In this article, following a general description of affinity adsorbents used for purification of proteinases, we overview a simple separation procedure for some serine proteinases and their inhibitors by way of a...

Because the consumption of soybean inhibitors of digestive enzymes in processed foods may have both beneficial and adverse health-related effects, reliable and rapid analytical methods for these inhibitors are needed. Monoclonal antibody–based sandwich enzyme-linked immunosorbent assays (ELISAs) were developed for the 2 major soybean protease inhibitors, the Kunitz trypsin inhibitor (KTI) and B...