Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The ...

The (-)-hyoscyamine, atropine and scopolamine (hyoscine) are three valuable tropane alkaloids while scopolamine is the most important member of this group for the pharmaceutical industry due to its higher demand compared to hyoscyamine and atropine. Scopolamine is an anticholinergic reagent with several therapeutic applications. In the current study, the hairy roots culture of Daturametel was u...

agrobacterium rhizogenes causes hairy root disease in plants. these hairy roots are ge-netically stable and grow rapidly. transformed hairy roots of hyoscyamus muticus in-duced by the bacterium can produce tropane alkaloids in trace amounts of intact plant tissues. in this research, in order to compare growth and biosynthetic stability of hairy roots with wild type ones, leaf and nodal segments...

Hyoscyamine-6beta-hydroxylase (H6H; EC 1.14.11.11) catalyses oxidative reactions in the biosynthetic pathway leading from hyoscyamine to the more pharmaceutically valuable tropane alkaloid scopolamine. The h6h gene encoding H6H from Hyoscyamus niger was introduced, under the control of the CaMV 35S promoter, into non-hyoscyamine-producing Nicotiana tabacum and hyoscyamine-producing Hyoscyamus m...

Two out of a group of 23 mares exposed to tef hay contaminated with Datura ferox (and possibly D. stramonium) developed colic. The 1st animal was unresponsive to conservative treatment, underwent surgery for severe intestinal atony and had to be euthanased. The 2nd was less seriously affected, responded well to analgesics and made an uneventful recovery. This horse exhibited marked mydriasis on...

Background: Since alkaloids are nitrogenous compounds, the availability of nitrogen (N) is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants. Nitrogen has been found to increase the content of alkaloids in some of the medicinal plants. Objective: The aim of this study was to compare the effects of nitrogen bio-fertilizers, conventional nitrogen fe...

This study was conducted to determine the effects of different culture times on root growth and accumulation secondary metabolites in adventitious roots from Hyoscyamus niger petioles. For this purpose, transferred media containing 1 mM methyl jasmonate (MeJA) were harvested at 11 times, starting day 30. After harvest, fresh dry weights index as parameters growth, well amounts tropane alkaloids...