UNLABELLED PREMISE OF THE STUDY Around the world, tropical glaciers and ice caps are retreating at unprecedented rates because of climate change. In at least one location, along the margin of the Quelccaya Ice Cap in southeastern Peru, ancient plant remains have been continually uncovered since 2002. We used genetic analysis to identify plants that existed at these sites during the mid-Holoc...

Aim. Development of an algorithm for creating ILP markers suitable breeding studies durum wheat (Triticum durum) and some other types cereals. Methods. Use classical bioinformatics tools to locate potential intron sites target genes in T. use as based on homology contigs rice (Oryza sativa) coding sequences. Analysis exon-intron structure homologous O. sativa, Hordeum vulgare, Aegilops tauschii...

Previous studies of the small Southern Hemisphere family Atherospermataceae have drawn contradictory conclusions regarding the number of transantarctic disjunctions and role of transoceanic dispersal in its evolution. Clarification of intergeneric relationships is critical to resolving (1) whether the two Chilean species, Laurelia sempervirens and Laureliopsis philippiana, are related to differ...

Information on the camel leptin gene when compared to other domestic livestock species is very limited. Hence, thepresent study was undertaken characterize of Indian dromedary (Camelus dromedarius).Five overlapping primer pairs were designed utilizing available Lama Glama sequence. Through theassembly, alignment, and editing five nucleotide sequences, a 2425 bp long sequence(Accession no. MT103...

Recent contributions from DNA sequences have revolutionized our concept of systematic relationships in angiosperms. However, parts of the angiosperm tree remain unclear. Previous studies have been based on coding or rDNA regions of relatively conserved genes. A phylogeny for basal angiosperms based on noncoding, fast-evolving sequences of the chloroplast genome region trnT-trnF is presented. Th...

Following a search of the Pyrococcus genomes for homologs of eukaryotic methylation guide small nucleolar RNAs, we have experimentally identified in Pyrococcus abyssi four novel box C/D small RNAs predicted to direct 2'-O-ribose methylations onto the first position of the anticodon in tRNALeu(CAA), tRNALeu(UAA), elongator tRNAMet and tRNATrp, respectively. Remarkably, one of them corresponds to...

Abstract. Partovi R, Iranbakhsh A, Sheidai M, Ebadi M. 2020. The use of DNA barcoding to avoid adulteration in olive plant leaf products. Asian J For 5: 42-47. leaves species Olea europeae, and O. europeae var. cuspidata have been used for medicinal perfused Iran. first control the blood pressure, while wild abortion by locals. Our preliminary inspection market revealed that these two are sold ...

Figure 3. Specific suppressor tRNAs reverse nonsense-mediated regulation. (A) Constructs A and C are described in the Fig. 1 legend. Construct J contains a UAG nonsense codon in place of the UAA nonsense in construct C but is otherwise identical. (B and C) Northern blot analysis of total cellular RNA isolated from HeLa cells co-transfected with the constructs (4 ␮g) shown in A, along with plasm...

Background: Human FIX (hFIX) gene transfer into hepatocytes has provided a novel approach for treatment of hemophilia B. To obtain an improved expression of hFIX, the functional hFIX-expressing plasmids with appropriate intron-derived fragments which facilitate transcription and promote an efficient 3′-end formation of mRNAs are required.Objectives: We ai...