DNA barcoding coupled high resolution melting (Bar-HRM) is an emerging method for species discrimination based on DNA dissociation kinetics. The aim of this work was to evaluate the suitability of different primer sets, derived from selected DNA regions, for Bar-HRM analysis of species in Croton (Euphorbiaceae), one of the largest genera of plants with over 1,200 species. Seven primer pairs wer...

BACKGROUND Species identification of living organisms by standard DNA sequences has been well-accepted. Consortium for the Barcode of Life (CBOL) recommends chloroplast regions rbcL and matK as the DNA barcodes for the land plants. This study aims to evaluate the feasibility and limitations of rbcL, matK, and 5 other commonly used regions as the DNA barcodes for the medicinal Gentiana and their...

A part of the tRNA(Leu)(UAA) gene containing a 240-nucleotide group I intron was amplified by PCR from cyanobacterium Synechococcus PCC 6301 genomic DNA. The pre-tRNA synthesized from the cloned PCR product was efficiently self-spliced in vitro under physiological conditions. The gene encoding the tRNA(Leu)(UAA), trnL-UAA, was isolated from a Synechococcus PCC 6301 genomic library and the nucle...

In this study, based on molecular biology techniques, the matK and trnL-F regions of ten Annonaceae species grown in Binh Chau-Phuoc Buu Nature Reserve, Vietnam were successfully amplified sequenced for first time. Along with other highly homologous DNA sequences from GenBank database, phylogeny studied was also established. By using alignment tool NCBI percentage identity among presented. The ...

The current research deals with molecular systematics study of the genus Eryngium (Apiaceae) in Iraq. included characteristics traits chloroplast matK & trnL-F gene that have reliable value separate and determinate from others genera Apiaceae family. result reaches to investigated phylogenetic relation species belongs Erymgium using five in-group E. campestre L., creticum Lam., billardieri ...

This study represents phylogenetic analyses of two woody polygonaceous genera Calligonum and Pteropyrum using both chloroplast fragment (trnL-F) and the nuclear ribosomal internal transcribed spacer (nrDNA ITS) sequence data. All inferred phylogenies using parsimony and Bayesian methods showed that Calligonum and Pteropyrum are both monophyletic and closely related taxa. They have no affinity w...

A cryptic invader of the genus Persicaria has recently increased in damper, northern parts La Palma and Gran Canaria Canary Islands (Spain) locally behaves as an invasive species. Examination historical herbarium specimens showed this species to be present since 1960s same probably applies Palma. Up now, had been assigned Old World weed P. maculosa. However, morphologically, these plants clearl...