Organelle-specific integral membrane proteins were identified by a novel strategy which gives rise to monospecific antibodies to these proteins as well as to the cDNA clones encoding them. A cDNA expression library was screened with a polyclonal antiserum raised against Triton X-114-extracted organelle proteins and clones were then grouped using antibodies affinity-purified on individual fusion...

The nucleotide sequence of the Treponema pallidum mcp2 gene was determined. mcp2 encodes a 45.8-kDa protein whose deduced amino acid sequence has significant homology with the C-terminal region of bacterial methyl-accepting chemotaxis proteins (MCPs). The Mcp2 N terminus lacks the hydrophobic transmembrane regions present in most MCPs. An Mcp2 fusion protein was strongly reactive with antibody ...

CONTEXT There is insufficient knowledge about the chondrocyte membranome and its molecular composition. OBJECTIVE To develop a Triton X-114 based separation technique using nanoLC-MS/MS combined with shotgun proteomics to identify chondrocyte membrane proteins. MATERIALS AND METHODS Articular chondrocytes from equine metacarpophalangeal joints were separated into hydrophobic and hydrophilic...

A simple, sensitive and selective cloud point extraction procedure is described for the preconcentration and atomic absorption spectrometric determination of Zn and Cd ions in water and biological samples, after complexation with 3,3’,3”,3’”-tetraindolyl (terephthaloyl) dimethane (TTDM) in basic medium, using Triton X-114 as nonionic surfactant. Detection limits of 3.0 and 2.0 μg L and quantifi...

The hyaluronate synthase complex was identified in plasma membranes from B6 cells. It contained two subunits of molecular masses 52 kDa and 60 kDa which bound the precursor UDP-GlcA in digitonin solution and partitioned into the aqueous phase, together with nascent hyaluronate upon Triton X-114 phase separation. The 52 kDa protein cross-reacted with poly- and monoclonal antibodies raised agains...

in this work, spherical pt nanometer-scale particles supported on the poly (m-toluidine)/triton x-100 film modified carbon nanotube paste electrode (pt/pmt (tx-100)/mcntpe) was used as a potent catalyst for electrooxidation of formaldehyde (hcho) in both 0.5 m h2so4 and 0.1 m naoh solutions. the obtained results showed that utilization of tx-100 as an additive during the electropolymerization p...