Glutathione S-transferase pi (GST pi) is an enzyme involved in cell protection against toxic electrophiles and products of oxidative stress. GST pi expression was studied in transgenic mice hybrids (B6-C3H) with symptoms of neurodegeneration harboring SOD1G93A (SOD1/+), Dync1h1 (Cra1/+) and double (Cra1/SOD1) mutations, at presymptomatic and symptomatic stages (age 70, 140, 365 days) using RT-P...

Rice blast, caused by the fungus Pyricularia oryzae L., is considered one of main threats to world rice production. The development resistant cultivars best and sustainable control alternatives. Plant breeding efforts have been accelerated genetic mapping (linkage associative) marker assisted selection. On other hand, genomic editing techniques, such as meganucleases (MNs), Zinc-finger nuclease...

Abstract Background Population suppression gene drive has been proposed as a strategy for malaria vector control. A CRISPR-Cas9-based transgene homing at the doublesex locus ( dsxF CRISPRh ) recently shown to increase rapidly in frequency in, and suppress, caged laboratory populations of mosquito Anopheles gambiae . Here, problem formulation, an initial step environmental risk assessment (ERA),...

Agricultural and environmental applications of modern biotechnology have spurred considerable controversy about their environmental risks and potential to alter the environmental impacts of other technologies and practices, such as pesticide use. The range of biotechnology products under development is expanding rapidly, and thus the potential for controversy over the environmental effects of b...

Two Citrus sinensis (L.) phosphomannose isomerase (PMI) genes, CsPMI1 and CsPMI2, were evaluated as novel selectable markers in mature citrus transformation. Transgenic shoots produced after transformation of Kuharske rootstock with each PMI construct selected on six treatments mannose sucrose. For CsPMI1, there no significant differences among the various sucrose for mean number positive (PS),...

We sought genetic evidence for the involvement of neuronal vascular endothelial growth factor (VEGF) in amyotrophic lateral sclerosis (ALS). Mice expressing human ALS mutant superoxide dismutase-1 (SOD1) were crossed with mice that overexpress VEGF in neurons (VEGF+/+). We report that SOD1(G93A)/VEGF+/+ double-transgenic mice show delayed motor neuron loss, delayed motor impairment, and prolong...

There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the...