Here, we present data showing the directed degradation of target proteins recognized by a specific nanobody in transgenic plants. Green fluorescent protein was depleted by a chimeric nanobody fused to a distinct F-box domain, which enables protein degradation via the ubiquitin proteasome pathway. This technique could thus be used to knock out other proteins of interest in planta using specific,...

E3 ligase plays an important role in the response to many environment stresses in plants. In our previous study, constitutive overexpression of an F-box protein gene TaFBA1 driven by 35S promoter improved the drought tolerance in transgenic tobacco plants, but the growth and development in transgenic plants was altered in normal conditions. In this study, we used stress-inducible promoter RD29A...

Terpenoids are important for plant survival and also possess biological properties that are beneficial to humans. Here, we describe the state of the art in terpenoid metabolic engineering, showing that significant progress has been made over the past few years. Subcellular targeting of enzymes has demonstrated that terpenoid precursors in subcellular compartments are not as strictly separated a...

37 Karpinksi. S. (1994) I’h.1). Thesis. Swedish University of Agricultural Sciences. limea Greer. S. and I’erham, K. N. (1986) Biochemistry 25. 2736-2742 Kunert, K. J., Cresswell. C. F., Schmidt. A.. Mullineaux, 1’. M. and Foyer, C. H. (1990) Arch. Hiochem. Hiophys. 282.233-238 Foyer. C. H.. Lelandais. M., Galap, C. and Kunert, K. J. (1991) I’lant I’hysiol. 97.803-872 Aono. M., Kubo. A., Saji9 ...

Arabidopsis thaliana was transformed with a gene encoding a nitroreductase (NTR, E.C.1.6.99.7) with activity against a wide range of nitroaromatic compounds. The gene was transferred from Escherichia coli by an Agrobacterium-mediated in planta method. The obtained seeds were sowed to produce T1 plants, and they were assayed for the integration of the transgene in the plant genome. Transgenic pl...

Although integrated pest management (IPM) strategies have been developed worldwide, further improvement of IPM effectiveness is required. The use of transgenic technology to create insect-resistant plants can offer a solution to the limited availability of highly insect-resistant cultivars. Commercially available insect-resistant transgenic crops show clear benefits for agriculture and there ar...

cell expansion and cell division are two main processes in plant growth and development. expansin proteins play a role key in cell expansion. these non-enzyme proteins involve in many growth and developmental processes. this protein affects drought tolerance. atexpb2 gene belongs to these proteins family. atexpb2 gene mainly expresses in the root tissue. in this study the expb2 gene, that was i...

a stable agrobacterium-mediated transformation system was established using bialaphos as the selective agent in soybeans [glycine max (l.) merr.]. the cotyledonary node explants of the soybean cultivar ‘ny-1001’ were inoculated with the agrobacterium tumefaciens strain eha105, harboring the vector pcambia3301 containing the gus gene as the reporter gene and the bar gene conferring bialaphos res...

During the past decade the pesticidal bacterium Bacillus thuringiensis has been the subject of intensive research. These efforts have yielded considerable data about the complex relationships between the structure, mechanism of action, and genetics of the organism's pesticidal crystal proteins, and a coherent picture of these relationships is beginning to emerge. Other studies have focused on t...

Concerns have been raised regarding the preparation of Figures 4, 5, and 6 and verification of the genotypes of the three transgenic plants—P15, P19, and HcPro—which were originally described in an article by Dunoyer et al. (2004, Plant Cell) that was retracted from the published literature. These concerns were rigorously investigated, the authors have been contacted, and efforts to fully addre...