The mechanism of translocation of matrix-targeted, cleavable preproteins across the mitochondrial outer membrane was studied using purified outer membrane vesicles. The N-terminal presequence interacts in a sequential and reversible fashion with two specific binding sites. The first one is provided by protease-sensitive receptors on the surface of the membrane (cis site); the second one is loca...

Mitochondrial outer- and inner-membrane fusion events are coupled in vivo but separable and mechanistically distinct in vitro, indicating that separate fusion machines exist in each membrane. Outer-membrane fusion requires trans interactions of the dynamin-related GTPase Fzo1, GTP hydrolysis, and an intact inner-membrane proton gradient. Inner-membrane fusion also requires GTP hydrolysis but di...

Utilization of membrane humidifiers is one of the methods commonly used to humidify reactant gases in polymer electrolyte membrane fuel cells (PEMFC). In this study, polymeric porous membranes with different compositions were prepared to be used in a membrane humidifier module and were employed in a humidification test. Three different neural network models were developed to investigate several...

Past experiments with reconstituted proteoliposomes, employing assays that infer membrane fusion from fluorescent lipid dequenching, have suggested that vacuolar SNAREs alone suffice to catalyze membrane fusion in vitro. While we could replicate these results, we detected very little fusion with the more rigorous assay of lumenal compartment mixing. Exploring the discrepancies between lipid-deq...

The present work reports the detailed B3LYP/6-311++G(d,p) study of most stable transand cisconfigurations photoisomerization in the core system of computational photochemistry-the 5,5'-disubstituted-1,1'-azobis (tetrazole) molecules. All computations were carried out in gas phase attemperature 293.15 K and pressure 1 atm. Firstly; the potential energy surface (PES) of the groundstate of the mol...

a pebax-nano zeolite x mixed matrix membrane was fabricated and operationally characterized using single gas (co2) permeation. x-ray diffraction (xrd) analysis was used to study the arrangement of polymer chains of mixed matrix membrane. the membranes were characterized by scanning electron microscopy (sem) to study cross-sectional morphology. the single gas permeability were carried out for ne...

The kinetics of Na/succinate cotransport across renal brush-borders was studied using membrane vesicles. Initial rates of succinate uptake (Js) were estimated from 1-s uptakes measured under voltage-clamped conditions. Lowering the external (cis) sodium concentration reduced the succinate Kt without affecting the Jsmax. Increasing the intravesicular (trans) sodium concentration reduced both Jsm...

Developing pea cotyledons contain functionally different vacuoles, a protein storage vacuole and a lytic vacuole. Lumenal as well as membrane proteins of the protein storage vacuole exit the Golgi apparatus in dense vesicles rather than in clathrin-coated vesicles (CCVs). Although the sorting receptor for vacuolar hydrolases BP-80 is present in CCVs, it is not detectable in dense vesicles. To l...

in the present study, the growth of phospholipids' micelles in canola oil by chemical pretreatment and their separation by using membrane technology was investigated. aqueous solutions of calcium chloride (1% w/w) and edta (150 mm) were added to canola oil miscella before ultrafiltration process. ultrafiltration was conducted by a flat sheet pvdf membrane with two different mwcos (50 kda, m116,...